一、产品简介：
谷胱甘肽还原酶（GR，EC 1.6.4.2）是在动植物中都有发现，是一类黄素蛋白氧化还原酶，催化氧化型谷胱甘肽(GSSG)还原成还原型谷胱甘肽（GSH），GSH / GSSG的比率越高，则越能清除氧化胁迫过程中产生的活性氧，因此GR酶活性高低是衡量氧化应激能力的一个重要指标。
本试剂盒采用Ellman方法，DTNB与GR中GSSG还原产生的GSH反应，生成黄色产物（TNB）。该产物在412nm出有最大吸收。TNB生成量和GR活性成线性正相关，可通过测定412nm处吸光值计算出谷胱甘肽还原酶（GR）的活性水平。该方法在可见光下测定，检测产物相对传统测试方法灵敏度高、测定物更稳定、可操作性更强。另外，改进样本的前处，加相应试剂先消除样本本身的GSH含量，以确保检测结果的精确度。
二、所需的仪器和用品：
酶标仪、96孔板、低温离心机、水浴锅、移液器、蒸馏水
1. Sugar Metabolism and Transcriptome Analysis Reveal Key Sugar Transporters during Camellia oleifera Fruit Development. International Journal of Molecular Sciences. IF=5.92
2. 刘梅，李祖然，张光群，王吉秀，祖艳群. 2020. 硫素对小花南芥体内含硫化合物与铅累积特征的影响.农业环境科学学报.
3. 牟凤利，杨京民，刘翠，刘才鑫，刘梅，陈建军，祖艳群，王吉秀.2021.野生小花南芥体内 AsA-GSH 循环对土壤 Cd、Pb 胁迫的响应.农业环境科学学报.
4. 孙晓莉.贾春燕.田寿乐.文燕.王金平.冉 昆.沈广宁. 2022. 外源甲基乙二醛对干旱胁迫下板栗幼苗的影响．应用生态学报.
5.Miaomiao Yao.2021.Exogenous glutathione alleviates chilling injury in postharvest bell pepper by modulating the ascorbate-glutathione (AsA-GSH) cycle.IF=7.514
6.Sihong Liu.2022.Combined effects of S-metolachlor and benoxacor on embryo development in zebrafish (Danio rerio).IF=7.129
7.Sihong Liu.2020.Developmental toxicity and transcriptome analysis of zebrafish (Danio rerio) embryos following exposure to chiral herbicide safener benoxacor.IF=6.551
8.Sihong Liu.2020.Assessing the toxicity of three “inert” herbicide safeners toward Danio rerio: Effects on embryos development.IF=4.872
9.Congcong Wang.2019.Characterization of the cellular effects and mechanism of arsenic trioxide-induced hepatotoxicity in broiler chickens.IF=3.067
10.Chen, Siting.2022.Overexpression of the intertidal seagrass J protein ZjDjB1 enhances tolerance to chilling injury.IF=2.496
11.Tian-shun Kou.2022.Exogenous glycine promotes oxidation of glutathione and restores sensitivity of bacterial pathogens to serum-induced cell death.IF=10.787
12.Chen, Siting.2022.Overexpression of the intertidal seagrass J protein ZjDjB1 enhances tolerance to chilling injury.IF=2.496
13.Yajing Wang.2022.Gaseous ozone treatment prolongs the shelf-life of fresh-cut kiwifruit by maintaining its ascorbic acid content.IF=6.056
14.Hang Yang.2023.Artemisia baimaensis allelopathy has a negative effect on the establishment of Elymus nutans artificial grassland in natural grassland.IF=2.734
15.Shufeng Wang.2023.Differentially-expressed genes related to glutathione metabolism and heavy metal transport reveals an adaptive, genotype-specific mechanism to Hg2+ exposure in rice (Oryza sativa L.).IF=9.988
16.Erting Fu.2023.Chitosan Reduces Damages of Strawberry Seedlings under High-Temperature and High-Light Stress.IF=3.949
17.Xingang Li.2023.GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.).IF=6.208
18.Meiqiong Tang.2023.Exogenous glutathione can alleviate chromium toxicity in kenaf by activating antioxidant system and regulating DNA methylation.IF=8.8
19.Rong Wu.2023.GsNAC2 gene enhances saline-alkali stress tolerance by promoting plant growth and regulating glutathione metabolism in Sorghum bicolor.IF=3
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
