一、产品简介： 4-香豆酸：辅酶 A 连接酶（4CL ，EC 6.2.1.12）是木质素生物合成的关键酶之一，位于 苯丙酸途径与木质素特异合成途径的转折点上，主要催化肉桂酸生成相应的肉桂酸辅酶 A 酯。该酶主要存在于高等植物、酵母和菌类中，研究该酶可以探讨多种生物细胞发育过程 中木质素沉积的代谢机理，为减少水果石细胞含量而提高其品质提供依据。 4CL 催化 4-香豆酸和 CoA 生成 4-香豆酸 CoA，在 333nm 下测 4-香豆酸 CoA 生成速率， 即可反映 4CL 活性。
二、所需的仪器和用品： 酶标仪、96 孔板（UV 板）、低温台式离心机、可调式移液器、研钵、冰和蒸馏水。
| 序号 | 论文标题 | 期刊 | IF | 原文链接 |
| 1 | Cinnamic acid treatment reduces the surface browning of fresh-cut taro | SCIENTIA HORTICULTURAE | 3.463 | https://www.sciencedirect.com/science/article/pii/S0304423821007202 |
| 2 | Melatonin Treatment Improves Postharvest Preservation and Resistance of Guava Fruit (Psidium guajava L.) | Foods | 5.561 | https://www.mdpi.com/2304-8158/11/3/262 |
| 3 | Shade-Tolerant Soybean Reduces Yield Loss by Regulating Its Canopy Structure and Stem Characteristics in the Maize–Soybean Strip Intercropping System | Frontiers in Plant Science | 6.627 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967133/ |
| 4 | Melatonin Maintains Fruit Quality and Reduces Anthracnose in Postharvest Papaya via Enhancement of Antioxidants and Inhibition of Pathogen Development | Antioxidants | 7.675 | https://www.mdpi.com/2076-3921/11/5/804 |
| 5 | Combined effects of high temperature and waterlogging on yield and stem development of summer maize | Crop Journal | 4.647 | https://www.sciencedirect.com/science/article/pii/S2214514122001957 |
| 6 | Dark septate endophyte Exophiala pisciphila promotes maize growth and alleviates cadmium toxicity | Frontiers in Microbiology | 6.064 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126344/ |
| 7 | Methyl jasmonate-loaded composite biofilm sustainably alleviates chilling lignification of loquat fruit during postharvest storage | FOOD CHEMISTRY | 8.8 | https://www.sciencedirect.com/science/article/pii/S0308814624002504 |
| 8 | Effects of UV-B Radiation Exposure on Transgenerational Plasticity in Grain Morphology and Proanthocyanidin Content in Yuanyang Red Rice | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 5.6 | https://www.mdpi.com/1422-0067/25/9/4766 |