一、产品简介：
谷胱甘肽过氧化物酶（GPx，EC 1.11.1.9）代表一种具有过氧化物酶活性的酶家族，在保护生物免受氧 化损伤中起重要作用。以往以过氧化氢为底物进行检测，则会受到分解过氧化氢的过氧化氢酶(Catalase)的干扰，本试剂盒提供的有机过氧化物试剂(Cum-OOH)不会被过氧化氢酶分解，因而可以特异地检测出谷胱甘肽过氧化物酶的活力。 因此本试剂盒可以定量检测总谷胱甘肽过氧化物酶。GSH 和 DTNB 反应产生黄色物质，后者在 412nm 下有最大吸收峰，而 GSH-Px 催化有机过氧化物试剂(Cum-OOH)氧化 GSH，使 GSH 量减少，GSH 量减少越多，反应混合液黄色越浅，则 GSH-Px 活性越大；反之，黄色越深，GSH-Px 活性越低。
二、所需的仪器和用品：
酶标仪、96孔酶标板、低温离心机、移液器、研钵。
1. 刘梅，李祖然，张光群，王吉秀，祖艳群. 2020. 硫素对小花南芥体内含硫化合物与铅累积特征的影响.农业环境科学学报.
2. 牟凤利，杨京民，刘翠，刘才鑫，刘梅，陈建军，祖艳群，王吉秀.2021.野生小花南芥体内 AsA-GSH 循环对土壤 Cd、Pb 胁迫的响应.农业环境科学学报.
3.Yue Wang.2021.Exogenous melatonin alleviates NO2 damage in tobacco leaves by promoting antioxidant defense, modulating redox homeostasis, and signal transduction.IF=10.588
4.Rui Jia.2022.Effects of Stocking Density on the Growth Performance, Physiological Parameters, Redox Status and Lipid Metabolism of Micropterus salmoides in Integrated Rice–Fish Farming Systems.IF=7.675
5.Sihong Liu.2022.Combined effects of S-metolachlor and benoxacor on embryo development in zebrafish (Danio rerio).IF=7.129
6.Sihong Liu.2020.Developmental toxicity and transcriptome analysis of zebrafish (Danio rerio) embryos following exposure to chiral herbicide safener benoxacor.IF=6.551
7.Yang Wang.2021.Protective Effect of Lactobacillus plantarum P8 on Growth Performance, Intestinal Health, and Microbiota in Eimeria-Infected Broilers.IF=5.64
8.Fuqi Wang.2021.Isorhamnetin, the xanthine oxidase inhibitor from Sophora japonica, ameliorates uric acid levels and renal function in hyperuricemic mice.IF=5.396
9.Cheng Wang.2022.Hepatoprotective effect of phillygenin on carbon tetrachloride-induced liver fibrosis and its effects on short chain fatty acid and bile acid metabolism.IF=4.36
10.Chen, Siting.2022.Overexpression of the intertidal seagrass J protein ZjDjB1 enhances tolerance to chilling injury.IF=2.496
11.Chen, Siting.2022.Overexpression of the intertidal seagrass 14-3-3 gene ZjGRF1 enhances the tolerance of transgenic Arabidopsis to salt and osmotic stress.IF=2.496
12.Dong Yin.2022.Effects of stocking density on the growth performance, mitophagy, endocytosis and metabolism of Cherax quadricarinatus in integrated rice–crayfish farming systems.IF=4.755
13.Hongbo Zhang.2022.Trx CDSP32-overexpressing tobacco plants improves cadmium tolerance by modulating antioxidant mechanism..IF=5.437
14.Mengqi Zhang.2023.Effects of light on growth, feeding rate, digestion, and antioxidation in juvenile razor clams Sinonovacula constricta.IF=5.135
15.Zhaojun Chen.2023.Protective effects of Fagopyrum dibotrys on oxidized oil-induced oxidative stress, intestinal barrier impairment, and altered cecal microbiota in broiler chickens.IF=4.014
16.Quanjun Zhan.2023.Effect of copper sulphate on Cryptocaryon irritans based on metabolome analysis.IF=2.58
17.Qianmin Lin.2023.Effect of Sodium Hyaluronate on Antioxidant and Anti-Ageing Activities in Caenorhabditis elegans.IF=5.561
18.Weixu Diao.2023.Effects of Stocking Density on the Growth Performance, Physiological Parameters, Antioxidant Status and Lipid Metabolism of Pelteobagrus fulvidraco in the Integrated Rice-Fish Farming System..IF=3.231
19.Rong Wu.2023.GsNAC2 gene enhances saline-alkali stress tolerance by promoting plant growth and regulating glutathione metabolism in Sorghum bicolor.IF=3
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
