一、产品简介：
抗坏血酸过氧化物酶（APX；EC 1.11.1.11)也称维生素 C 过氧化物酶，是植物细胞中防御外界氧化胁迫和植物本身活性氧代谢的重要抗氧化酶类，在降低 H2O2对植物细胞产生氧化损伤方面起关键作用。抗坏血酸过氧化物酶（APX）首先与 H2O2 形成中间复合物，中间复合物接着氧化 AsA，因此 APX 与 AsA 具有一定的负相关性，通过测定 AsA 氧化速率，来计算 APX 酶活性。
二、所需仪器和用品：
酶标仪、96孔酶标板、低温离心机、移液枪、研钵、冰和蒸馏水。
1. 牟凤利，杨京民，刘翠，刘才鑫，刘梅，陈建军，祖艳群，王吉秀.2021.野生小花南芥体内 AsA-GSH 循环对土壤 Cd、Pb 胁迫的响应.农业环境科学学报.
2. 孙晓莉.贾春燕.田寿乐.文燕.王金平.冉 昆.沈广宁. 2022. 外源甲基乙二醛对干旱胁迫下板栗幼苗的影响．应用生态学报.
3.Miaomiao Yao.2021.Exogenous glutathione alleviates chilling injury in postharvest bell pepper by modulating the ascorbate-glutathione (AsA-GSH) cycle.IF=7.514
4.Ali Raza.2022.Mechanistic Insights Into Trehalose-Mediated Cold Stress Tolerance in Rapeseed ( Brassica napus L.) Seedlings.IF=5.754
5.Sundas Saher Mehmood.2021.Integrated analysis of transcriptomics and proteomics provides insights into the molecular regulation of cold response in Brassica napus.IF=5.545
6.Liu, Xiaohui.2021.Study on browning mechanism of fresh-cut eggplant (Solanum melongena L.) based on metabolomics, enzymatic assays and gene expression.IF=4.38
7.Lei Liu.2020.Exogenous allantoin improves the salt tolerance of sugar beet by increasing putrescine metabolism and antioxidant activities.IF=3.72
8.Jiajia Wang.2022.LEAF TIP RUMPLED 1 Regulates Leaf Morphology and Salt Tolerance in Rice.IF=6.208
9.Xie Wanfeng.2022.Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infection.IF=5.26
10.Qibin Wu.2023.Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance.IF=8.025
11.Jinxin Li.2023.Artemisia argyi essential oil exerts herbicidal activity by inhibiting photosynthesis and causing oxidative damage.IF=6.449
12.Jiao Du.2023.A prophage-encoded effector from “Candidatus Liberibacter asiaticus” targets ASCORBATE PEROXIDASE6 in citrus to facilitate bacterial infection.IF=5.52
13.Hang Yang.2023.Artemisia baimaensis allelopathy has a negative effect on the establishment of Elymus nutans artificial grassland in natural grassland.IF=2.734
14.Erting Fu.2023.Chitosan Reduces Damages of Strawberry Seedlings under High-Temperature and High-Light Stress.IF=3.949
15.Xingang Li.2023.GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.).IF=6.208
16.Meiqiong Tang.2023.Exogenous glutathione can alleviate chromium toxicity in kenaf by activating antioxidant system and regulating DNA methylation.IF=8.8
17.Cun Yu.2023.Trichoderma longibrachiatum Inoculation Improves Drought Resistance and Growth of Pinus massoniana Seedlings through Regulating Physiological Responses and Soil Microbial Community.IF=4.7
18.Yutan Chu.Melatonin Alleviates Antimony Toxicity by Regulating the Antioxidant Response and Reducing Antimony Accumulation in Oryza sativa L.Antioxidants.IF=7
19.Peng Mu.Genomic features of a plant growth-promoting endophytic Enterobacter cancerogenus JY65 dominant in microbiota of halophyte Suaeda salsa.plant and soil.IF=4.9
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
