一、产品简介:
在碱性溶液中,凡分子中含二个或二个以上酰胺基(—CO-NH2)或与此相似基团的化合物均与二价铜离子作用,络合物呈紫色,这一反应称双缩脲反应。蛋白质分子含有众多肽键(—CONH—),可发生双缩脲反应,且呈色强度在一定浓度范围内与蛋白质含量成正比,经光谱扫描540nm为最大吸波长。
二、所需的仪器和用品:
可见分光光度计、1mL 玻璃比色皿(光径1cm)、台式离心机、恒温水浴锅、移液器。
1.《Evaluating physiological changes of grass and semishrub species with seasonality for understanding the process of shrub encroachment in semiarid grasslands》.2021.《Functional Plant Biology》.IF=3.1
2.《Transcriptome and Co-expression Network Analyses Reveal Differential Gene Expression and Pathways in Response to Severe Drought Stress in Peanut (Arachis hypogaea L.)》.2021.《Frontiers in Genetics》.IF=4.6
3.《Resveratrol improves the iron defciency adaptation of Malus baccata seedlings by regulating iron absorption》.2021.《BMC Plant Biol》.IF=4.2
4.《Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stage》.2021.《Field Crops Research》.IF=5.224
5.Qikang Wang.Differences in seed characteristics, germination and seedling growth of Suaeda salsa grown in intertidal zone and on saline inland.Frontiers in Plant Science.IF=5.6
6.Luo Zhenhua.Physiological and transcriptomic analysis reveals the potential mechanism of Morinda officinalis How in response to freezing stress.bmc plant biology.IF=5.3
1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?
答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。
2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?
答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;
微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。
3、问:分光法试剂盒与微板法试剂盒是否能通用?
答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!