一、产品简介：
总黄酮，即黄酮类化合物；是植物重要的一类次生代谢产物，具有有较强的抗氧化活性，可捕捉活性氧自由基，降低氧化伤害，在果实中影响其色泽和风味；对植物的抗逆性和抗病虫害方面有重要作用。
本试剂盒采用 NaNO 2 -Al(NO 3 ) 3 -NaOH 显色法测定黄酮总含量，即在碱性亚硝酸盐溶液中，黄酮类化合物与铝离子形成在 510nm 处有特征吸收峰的红色络合物，测定反应产物在 510nm 处的吸光值，即可计算样品中总黄酮含量。
二、所需仪器和用品：
可见分光光度计、1mL玻璃比色皿（光径1cm）、粉碎仪、筛子、60%乙醇、离心机、蒸馏水。
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Yanhui Xiao.2021.Cinnamic acid treatment reduces the surface browning of fresh-cut taro.IF=3.463
Yan Wang.2022.Beneficial effects of cadmium on plant defense of an invasive plant.IF=6.208
Xie Wanfeng.2022.Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infection.IF=5.26
Fei Ren.2023.Deciphering Microbial Diversity and Functional Codes of Traditional Fermented Whole Grain Tianpei from Typical Regions of China.IF=5.123
Liu, Yun-Shan.2023.Asymmetric inter-specific competition between invasive Phytolacca americana and its native congener.IF=1.99
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Yuanyuan Li.2023.Preparation of Thifluzamide Polylactic Acid Glycolic Acid Copolymer Microspheres and Its Effect on the Growth of Cucumber Seedlings.IF=5.6
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1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
