一、产品简介:
木质素是一种含有羟基和甲氧基的高分子芳香族化合物,是苯丙烷的衍生物,作为植物的化学成分与纤维素及半纤维素共同形成植物体骨架,大大提高了细胞壁的机械强度,为植物提供支持,适应环境,抗虫病害和抗倒伏的生理功能,同时,在木质素合成过程中产生的一些前体物质酚类和自由基可以破坏原菌相关酶类的生物活性与细胞膜的通透性,从而使植物有一定的自我防御能力。
本试剂盒采用乙酰化法,使木质素中的酚羟基发生乙酰化,其在280nm处有特征吸收峰,280nm的吸光值高低与木质素含量正相关。
二、所需的仪器和用品:
酶标仪、96孔UV板、低温离心机、水浴锅、移液器、蒸馏水、冰乙
1. Fuhua Fan. 2021. Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings. International Journal of Molecular Sciences.IF=5.9
2. Bin Cheng. 2022. Shade-Tolerant Soybean Reduces Yield Loss by Regulating Its Canopy Structure and Stem Characteristics in the Maize–Soybean Strip Intercropping System. Frontiers in Plant Science.IF=5.75
3. Xinyue Bi. 2022. A novel strategy for improving watermelon resistance to cucumber green mottle mosaic virus by exogenous boron application. Molecular Plant Pathology IF=5.66
4.Jiemeng Tao.2022.Metagenomic insight into the microbial degradation of organic compounds in fermented plant leaves.IF=8.431
5.Bin Cheng.2022.Shade-Tolerant Soybean Reduces Yield Loss by Regulating Its Canopy Structure and Stem Characteristics in the Maize–Soybean Strip Intercropping System..IF=6.627
6.Hong Yang.2022.Wheat Escapes Low Light Stress by Altering Pollination Types.IF=6.627
7.Kebin Yang.2022.Nitrogen fertilization in bamboo forest accelerates the shoot growth and alters the lignification process in shoots.IF=6.449
8.Fuhua Fan.2021.Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings.IF=5.924
9.Xinyue Bi.2022.A novel strategy for improving watermelon resistance to cucumber green mottle mosaic virus by exogenous boron application.IF=5.663
10.Feng Xiao.2022.Trade-off between shade tolerance and chemical resistance of invasive Phytolacca americana under different light levels compared with its native and exotic non-invasive congeners.IF=5.545
11.Yanhui Xiao.2021.Cinnamic acid treatment reduces the surface browning of fresh-cut taro.IF=3.463
12.Xinyue Bi.2022.Suppression of Cucumber Green Mottle Mosaic Virus Infection by Boron Application: From the Perspective of Nutrient Elements and Carbohydrates.IF=5.895
13.Qian Li.2022.Pb4CL2 Inducing Lignin Accumulation in Superficial Scald ‘Chili’ (Pyrus bretschneideri) Pear Fruit.IF=3.949
14.Xiaoshuang Li.2022.The ScAPD1-like gene from the desert moss Syntrichia caninervis enhances resistance to Verticillium dahliae via phenylpropanoid gene regulation.IF=7.091
15.Qixian Wu.2022.Multiple metabolomics comparatively investigated the pulp breakdown of four dragon fruit cultivars during postharvest storage.IF=7.425
16.Jia Wang.2023.Cell wall remodeling in woody tung oil tree root xylem contributes to deterrence of the Fusarium wilt pathogen.IF=6.449
17.Liu, Yun-Shan.2023.Asymmetric inter-specific competition between invasive Phytolacca americana and its native congener.IF=1.99
18.Bei Zhang.2023.Ectopic Expression of FvVND4c Promotes Secondary Cell Wall Thickening and Flavonoid Accumulation in Fragaria vesca.IF=6.208
19.Lei Wang.2023.Dark septate endophyte Exophiala pisciphila promotes maize growth and alleviates cadmium toxicity.IF=6.064
20.Yan Wang.2023.Comparative physiological and transcriptomic analyses provide insights into fruit softening in Chinese cherry [Cerasus pseudocerasus (Lindl.) G.Don].IF=5.6
1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?
答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。
2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?
答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;
微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。
3、问:分光法试剂盒与微板法试剂盒是否能通用?
答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!