一、产品简介：
果胶酶是指分解果胶的多种酶，主要包括多聚半乳糖醛酸酶(PG)，果胶裂解酶(PL)，果胶甲酯酶(PME)和原果胶酶，贮藏过程中起作用的主要是PG。所以该酶在食品贮藏保鲜和植物抗病性等领域具有较高的研究价值。
果胶在多聚半乳糖醛酸酶(PG)作用下，能水解产生带有具有还原性醛基的半乳糖醛酸。与DNS试剂反应生成红棕色物质，在540nm有特征吸收峰，测定540nm处吸光值变化可计算得多聚半乳糖醛酸酶活性。
二、所需的仪器和用品：
酶标仪、96孔板、台式离心机、天平、可调式移液器、恒温水浴锅、研钵、冰。
1. Jing Yang.2021. Inhibitory effects and mechanisms of vanillin on gray mold and black rot of cherry tomatoes. Pesticide Biochemistry and Physiology. IF=3.9
2.Yujie Song.2022.Enzymes and microorganisms jointly promote the fermentation of rapeseed cake.IF=6.59
3.Yu-Xuan Wu.2022.Inhibitory effect and mechanism of action of juniper essential oil on gray mold in cherry tomatoes.IF=6.064
4.Yun-Ze Chen.2022.Antifungal Activity of 6-Methylcoumarin against Valsa mali and Its Possible Mechanism of Action.IF=5.724
5.Na Li.2023.Phytic acid is a new substitutable plant-derived antifungal agent for the seedling blight of Pinus sylvestris var. mongolica caused by Fusarium oxysporum..IF=4.966
6.Liu-ya Wang.2023.Effect of chitosan-ascorbic acid composite coating on postharvest quality of Custard apple (Annona squamosa).IF=4.885
7.Siqiang Wang.2023.Transcriptome analysis reveals key metabolic pathways and gene expression involving in cell wall polysaccharides-disassembling and postharvest fruit softening in custard apple (Annona squamosa L.).IF=8.025
8.Wang Yuan.2023.Polygalacturonase gene family analysis identifies FcPG12 as a key player in fig (Ficus carica L.) fruit softening.IF=5.3
9.Wenqi Li.2023.Identification, pathogenic mechanism and control of Rhizopus oryzae causing postharvest fruit rot in pumpkin.IF=7
10.Yan Wang.2023.Comparative physiological and transcriptomic analyses provide insights into fruit softening in Chinese cherry [Cerasus pseudocerasus (Lindl.) G.Don].IF=5.6
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
