一、产品简介：
当生物体遭受外界胁迫时，生物体内超氧阴离子等活性氧大量产生和积累，可作为生物体氧化胁迫的信号。因此在逆境条件下生物体内超氧阴离子自由基的产生，可间接反映组织细胞受损状况和抗性强弱。超氧阴离子（O2-）与羟胺反应产生NO2－，NO2－在对氨基苯磺酸和α-萘胺作用下，生成粉红色的偶氮染料，该染料在540nm处有最大光吸收，根据A540值可计算出样品中的O2-的含量。
二、所需的仪器和用品：
酶标仪、96孔板、天平、水浴锅、离心机、研钵、可调式移液器、冰、蒸馏水。
1. Mingyang He. 2021. α-Lipoic acid treatment alleviates postharvest pericarp browning of litchi fruit by regulating antioxidant ability and energy metabolism.Postharvest Biology and Technology. IF=5.5
2. Ze Yun. 2021. Effects of hydrogen water treatment on antioxidant system of litchi fruit during the pericarp browning. Food Chemistry. IF=7.5
3. 孙晓莉.贾春燕.田寿乐.文燕.王金平.冉 昆.沈广宁. 2022. 外源甲基乙二醛对干旱胁迫下板栗幼苗的影响．应用生态学报.
4.Chang Wang.2022.Red light regulates metabolic pathways of soybean hypocotyl elongation and thickening.IF=6.028
5.Mingyang He.2021.α-Lipoic acid treatment alleviates postharvest pericarp browning of litchi fruit by regulating antioxidant ability and energy metabolism.IF=5.537
6.Yan Jia.2021.Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stage.IF=5.224
7.Wang Yue.2020.Elevated NO2 damages the photosynthetic apparatus by inducing the accumulation of superoxide anions and peroxynitrite in tobacco seedling leaves.IF=4.872
8.Baizhao Ren.2022.Responses of photosynthetic characteristics and leaf senescence in summer maize to simultaneous stresses of waterlogging and shading.IF=4.647
9.Liu, Xiaohui.2021.Study on browning mechanism of fresh-cut eggplant (Solanum melongena L.) based on metabolomics, enzymatic assays and gene expression.IF=4.38
10.Thwin Myo.2020.Ectopic overexpression of a cotton plastidial Na + transporter GhBASS5 impairs salt tolerance in Arabidopsis via increasing Na + loading and accumulation.IF=3.39
11.Chen, Siting.2022.Overexpression of the intertidal seagrass J protein ZjDjB1 enhances tolerance to chilling injury.IF=2.496
12.Yanfei Wu.2022.Valeric acid delays aril breakdown of longan (Dimocarpus longan Lour.) fruit in relation to the regulation of histone deacetylase activity.IF=6.056
13.Qiqi Chen.2022.Biocontrol activity and action mechanism of Bacillus velezensis strain SDTB038 against Fusarium crown and root rot of tomato.IF=6.064
14.Chen Siting.2023.Overexpression of Zostera japonica J protein gene ZjDjB1 in Arabidopsis enhanced the tolerance to lead stress.IF=2.742
15.Chen, Siting.2022.Overexpression of the intertidal seagrass 14-3-3 gene ZjGRF1 enhances the tolerance of transgenic Arabidopsis to salt and osmotic stress.IF=2.496
16.Yuhang Chen.2022.Preharvest debagging alleviates external CO2 injury of ‘Fuji’ apple during storage by improving antioxidant capacity and energy status.IF=6.751
17.Zhengwei Liang.2022.An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng.IF=6.59
18.Xuejing Cao.2022.Grape BES1 transcription factor gene VvBES1-3 confers salt tolerance in transgenic Arabidopsis.IF=3.913
19.Jiao Du.2023.A prophage-encoded effector from “Candidatus Liberibacter asiaticus” targets ASCORBATE PEROXIDASE6 in citrus to facilitate bacterial infection.IF=5.52
20.Hang Yang.2023.Artemisia baimaensis allelopathy has a negative effect on the establishment of Elymus nutans artificial grassland in natural grassland.IF=2.734
21.Erting Fu.2023.Chitosan Reduces Damages of Strawberry Seedlings under High-Temperature and High-Light Stress.IF=3.949
22.Chen Xuan.2023.In situ forming ROS-scavenging hybrid hydrogel loaded with polydopamine-modified fullerene nanocomposites for promoting skin wound healing.IF=9.429
23.Xiaomei Li.2023.Characterization of Chlorophyll Fluorescence and Antioxidant Defense Parameters of Two Gracilariopsis lemaneiformis Strains under Different Temperatures.IF=4.658
24.Yandong Xia.2023.Antagonistic Activity and Potential Mechanisms of Endophytic Bacillus subtilis YL13 in Biocontrol of Camellia oleifera Anthracnose.IF=3.282
25.Song Jianfei.2023.MhCLC-c1, a Cl channel c homolog from Malus hupehensis, alleviates NaCl-induced cell death by inhibiting intracellular Cl– accumulation.IF=5.3
26.Zhu Jiawei.2023.Isolation of three MiDi19-4 genes from mango, the ectopic expression of which confers early flowering and enhances stress tolerance in transgenic Arabidopsis.IF=4.3
27.Peng Mu.Genomic features of a plant growth-promoting endophytic Enterobacter cancerogenus JY65 dominant in microbiota of halophyte Suaeda salsa.plant and soil.IF=4.9
28.Y. Liang.Sophora tonkinensis: response and adaptation of physiological characteristics, functional traits, and secondary metabolites to drought stress.IF=3.9
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
