一、产品简介：
植物体内游离脯氨酸含量在一定程度上反映了植物的抗逆性，抗旱性强的品种往往积累 较多的脯氨酸。因此测定脯氨酸含量可以作为抗旱育种的生理指标。另外，由于脯氨酸亲 水性极强，能稳定原生质胶体及组织内的代谢过程，因而能降低凝固点，有防止细胞脱水 的作用。在低温条件下，植物组织中脯氨酸含量增加，可提高植物的抗寒性，因此，亦可作为抗寒育种的生理指标。
当用磺基水杨酸提取植物样品时，脯氨酸便游离于磺基水杨酸的溶液中，然后与酸性茚三酮加热反应后形成红色物质，该红色物质在 520nm 处有最大吸收峰，进而通过比色法测定植物体内游离脯氨酸的含量。
二、所需的仪器和用品：
可见分光光度计、1mL 玻璃比色皿（光径 1cm）、恒温水浴锅、台式离心机、可调式移液器、冰醋酸、研钵、冰
1.《Evaluating physiological changes of grass and semishrub species with seasonality for understanding the process of shrub encroachment in semiarid grasslands》.2021.《Functional Plant Biology》.IF=3.1
2.《Resveratrol improves the iron defciency adaptation of Malus baccata seedlings by regulating iron absorption》.2021.《BMC Plant Biol》.IF=4.2
3.Ming Gao.2022.Sex-specific physiological and biochemical responses of Litsea cubeba under waterlogging stress.IF=6.028
4.Yi Zhang.2022.Analysis of Lhcb gene family in rapeseed (Brassica napus L.) identifies a novel member “BnLhcb3.4” modulating cold tolerance.IF=6.028
5.Tingting Li.2021.Resveratrol Alleviates the KCl Salinity Stress of Malus hupehensis Rhed.IF=5.754
6.Ali Raza.2022.Mechanistic Insights Into Trehalose-Mediated Cold Stress Tolerance in Rapeseed ( Brassica napus L.) Seedlings.IF=5.754
7.Raza Ali.2021.Integrated Analysis of Metabolome and Transcriptome Reveals Insights for Cold Tolerance in Rapeseed (Brassica napus L.).IF=5.754
8.Dao-Jun Guo.2021.Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5.IF=5.754
9.Yan Jia.2021.Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stage.IF=5.224
10.Shifa Xiong.2022.Effects of Drought Stress and Rehydration on Physiological and Biochemical Properties of Four Oak Species in China.IF=4.658
11.Ying Zhou.2022.Proteomic Investigation of Molecular Mechanisms in Response to PEG-Induced Drought Stress in Soybean Roots.IF=4.658
12.Xiaodong Zheng.2020.Exogenous Strigolactones alleviate KCl stress by regulating photosynthesis, ROS migration and ion transport in Malus hupehensis Rehd.IF=3.72
13.Liu Yang.2020.Evaluating physiological changes of grass and semishrub species with seasonality for understanding the process of shrub encroachment in semiarid grasslands.IF=2.617
14.Yigong Zhang.2022.Structure, development, and the salt response of salt bladders in Chenopodium album L..IF=6.627
15.Zhanyu Chen.2022.Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in Glycine max (L.) Merr.IF=6.208
16.Qing-Qing Shen.2023.The SsWRKY1 transcription factor of Saccharum spontaneum enhances drought tolerance in transgenic Arabidopsis thaliana and interacts with 21 potential proteins to regulate drought tolerance in S. spontaneum.IF=5.437
17.Chen Siting.2023.Overexpression of Zostera japonica J protein gene ZjDjB1 in Arabidopsis enhanced the tolerance to lead stress.IF=2.742
18.Sun, Zhijuan.2022.Brassinolide alleviates Fe deficiency-induced stress by regulating the Fe absorption mechanism in Malus hupehensis Rehd.IF=4.964
19.Hong Zhu.2021.The sweetpotato β-amylase gene IbBAM1.1 enhances drought and salt stress resistance by regulating ROS homeostasis and osmotic balance.IF=4.27
20.Hong Zhu.2022.The Sweetpotato Voltage-Gated K+ Channel β Subunit, KIbB1, Positively Regulates Low-K+ and High-Salinity Tolerance by Maintaining Ion Homeostasis.IF=4.141
21.Juan Wang.2022.Transcriptome and Metabolome Analyses Reveal Complex Molecular Mechanisms Involved in the Salt Tolerance of Rice Induced by Exogenous Allantoin.IF=7.675
22.Ting-ting Lu.2023.Overexpression of mango MiRZFP34 confers early flowering and stress tolerance in transgenic Arabidopsis.IF=4.342
23.Xiaoning Fan.2023.A module centered on the transcription factor Msn2 from arbuscular mycorrhizal fungus Rhizophagus irregularis regulates drought stress tolerance in the host plant.IF=9.4
24.Hong Zhu.2023.The Sweet Potato K+ Transporter IbHAK11 Regulates K+ Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion Homeostasis.IF=4.5
25.Zhijuan Sun.2023.Melatonin enhances KCl salinity tolerance by maintaining K+ homeostasis in Malus hupehensis.IF=13.8
26.Zhao Meicheng.2023.The osmotic stress–activated receptor-like kinase DPY1 mediates SnRK2 kinase activation and drought tolerance in Setaria.IF=11.6
27.Junliang Li.2023.Unique Features of the m6A Methylome and Its Response to Salt Stress in the Roots of Sugar Beet (Beta vulgaris).IF=5.6
28.Meng Yang.2023.Transcriptomic Response to Drought Stress in Populus davidiana Dode.IF=2.9
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
