一、产品简介：
苯丙氨酸解氨酶（PAL，EC 4.3.1.24）是催化苯丙烷类代谢途径第一步反应的酶，也是这个途径的关键酶和限速酶，与一些重要的次生物质如木质素、异黄酮类植保素、黄酮类色素等合成密切相关，另外研究发现许多植物在遭受寒冷、伤害、紫外辐射时，植物的防卫体系特别是苯丙烷类代谢被激活，PAL活性迅速上升，因此PAL活性也可以作为植物抗逆境能力的一个生理指标。
本试剂盒根据苯丙氨酸解氨酶PAL催化L-苯丙氨酸裂解为反式肉桂酸和氨，反式肉桂酸在290nm处有最大吸收值，通过测定吸光值升高速率计算PAL活性。
二、所需的仪器和用品：
酶标仪、96孔板(UV板)、水浴锅、离心机、可调式移液器、研钵、冰和蒸馏水。
【注】：普通酶标板只能透过可见光，不能透过紫外光，检测波长小于340nm必须使用UV板
1. Bin Cheng.2020. Effects of Multiple Planting Densities on Lignin Metabolism and Lodging Resistance of the Strip Intercropped Soybean Stem. Agronomy. IF=3.4
2. Bin Cheng. 2022. Shade-Tolerant Soybean Reduces Yield Loss by Regulating Its Canopy Structure and Stem Characteristics in the Maize–Soybean Strip Intercropping System. Frontiers in Plant Science. IF=5.75
3.Yang Kebin.2021.A regulatory network driving shoot lignification in rapidly growing bamboo.IF=8.34
4.Silin Fan.2022.Melatonin Maintains Fruit Quality and Reduces Anthracnose in Postharvest Papaya via Enhancement of Antioxidants and Inhibition of Pathogen Development.IF=7.675
5.Bin Cheng.2022.Shade-Tolerant Soybean Reduces Yield Loss by Regulating Its Canopy Structure and Stem Characteristics in the Maize–Soybean Strip Intercropping System..IF=6.627
6.Kebin Yang.2022.Nitrogen fertilization in bamboo forest accelerates the shoot growth and alters the lignification process in shoots.IF=6.449
7.Yan Wang.2022.Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng.IF=6.064
8.Hubiao Jiang.2021.Effect of the Nanoparticle Exposures on the Tomato Bacterial Wilt Disease Control by Modulating the Rhizosphere Bacterial Community.IF=5.924
9.Yangyang Sun.2022.Phenylpropanoid metabolism in relation to peel browning development of cold-stored ‘Nanguo’ pears.IF=4.729
10.Caiyun Xiong.2022.Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred Lines.IF=6.208
11.Jingyi Shao.2022.Combined effects of high temperature and waterlogging on yield and stem development of summer maize.IF=4.647
12.Xie Wanfeng.2022.Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infection.IF=5.26
13.Jinxin Liu.2023.Biocontrol ability and action mechanism of Bacillus amyloliquefaciens Baf1 against Fusarium incarnatum causing fruit rot in postharvest muskmelon (cv. Yugu) fruit.IF=6.056
14.Lei Sun.Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporum.Frontiers in Microbiology.IF=5.2
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
