0512-66188056

产品中心

技术服务 试剂盒开发

苯丙氨酸解氨酶(PAL)试剂盒

货号:G0114W
规格:96样
价格:920
说明书:点击下载
提示:官网说明书如与试剂盒内纸质版有出入,请以纸质版为准!

  • 产品简介
  • 已发文章
  • 产品问答

一、产品简介:

苯丙氨酸解氨酶(PAL,EC 4.3.1.24)是催化苯丙烷类代谢途径第一步反应的酶,也是这个途径的关键酶和限速酶,与一些重要的次生物质如木质素、异黄酮类植保素、黄酮类色素等合成密切相关,另外研究发现许多植物在遭受寒冷、伤害、紫外辐射时,植物的防卫体系特别是苯丙烷类代谢被激活,PAL活性迅速上升,因此PAL活性也可以作为植物抗逆境能力的一个生理指标。

本试剂盒根据苯丙氨酸解氨酶PAL催化L-苯丙氨酸裂解为反式肉桂酸和氨,反式肉桂酸在290nm处有最大吸收值,通过测定吸光值升高速率计算PAL活性。

二、所需的仪器和用品

酶标仪96孔板(UV板)、水浴锅、离心机、可调式移液器、研钵、冰蒸馏水

【注】:普通酶标板只能透过可见光,不能透过紫外光,检测波长小于340nm必须使用UV板

1. Bin Cheng.2020. Effects of Multiple Planting Densities on Lignin Metabolism and Lodging Resistance of the Strip Intercropped Soybean Stem. Agronomy. IF=3.4


2. Bin Cheng. 2022. Shade-Tolerant Soybean Reduces Yield Loss by Regulating Its Canopy Structure and Stem Characteristics in the Maize–Soybean Strip Intercropping System. Frontiers in Plant Science. IF=5.75


3.Yang Kebin.2021.A regulatory network driving shoot lignification in rapidly growing bamboo.IF=8.34


4.Silin Fan.2022.Melatonin Maintains Fruit Quality and Reduces Anthracnose in Postharvest Papaya via Enhancement of Antioxidants and Inhibition of Pathogen Development.IF=7.675


5.Bin Cheng.2022.Shade-Tolerant Soybean Reduces Yield Loss by Regulating Its Canopy Structure and Stem Characteristics in the Maize–Soybean Strip Intercropping System..IF=6.627


6.Kebin Yang.2022.Nitrogen fertilization in bamboo forest accelerates the shoot growth and alters the lignification process in shoots.IF=6.449


7.Yan Wang.2022.Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng.IF=6.064


8.Hubiao Jiang.2021.Effect of the Nanoparticle Exposures on the Tomato Bacterial Wilt Disease Control by Modulating the Rhizosphere Bacterial Community.IF=5.924


9.Yangyang Sun.2022.Phenylpropanoid metabolism in relation to peel browning development of cold-stored ‘Nanguo’ pears.IF=4.729


10.Caiyun Xiong.2022.Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred Lines.IF=6.208


11.Jingyi Shao.2022.Combined effects of high temperature and waterlogging on yield and stem development of summer maize.IF=4.647


12.Xie Wanfeng.2022.Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infection.IF=5.26


13.Jinxin Liu.2023.Biocontrol ability and action mechanism of Bacillus amyloliquefaciens Baf1 against Fusarium incarnatum causing fruit rot in postharvest muskmelon (cv. Yugu) fruit.IF=6.056


14.Lei Sun.Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporum.Frontiers in Microbiology.IF=5.2



1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?

     答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。

          “24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。


2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?

     答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;

           微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。


3、问:分光法试剂盒与微板法试剂盒是否能通用?

     答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!