一、产品简介：

脂肪酶(EC 3.1.1.3)是一种特殊的酯键水解酶，能催化天然油脂水解,，在食品、医药、洗涤剂和皮革等许多工业领域中都有广泛的应用,

本试剂盒提供一种简单、快速的检测方法，以对硝基苯酚酯作为底物，脂肪酶水解底物产生具有颜色的对硝基苯酚，在405nm波长下测定其吸光值，即可得出脂肪酶活力。

二、所需的仪器和用品：

酶标仪、96孔板、研钵、低温离心机、可调式移液枪、冰和蒸馏水。

1. Yangyi Hao. 2021. Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves. Animals. IF=2.75

2. Yangyi Hao. 2021. Effects of Age, Diet CP, NDF, EE, and Starch on the Rumen Bacteria Community and Function in Dairy Cattle. Microorganisms. IF=3.94

3.Silin Fan.2022.Melatonin Maintains Fruit Quality and Reduces Anthracnose in Postharvest Papaya via Enhancement of Antioxidants and Inhibition of Pathogen Development.IF=7.675

4.Silin Fan.2022.Melatonin Treatment Improves Postharvest Preservation and Resistance of Guava Fruit (Psidium guajava L.).IF=5.561

5.Yangyi Hao.2021.Effects of Age, Diet CP, NDF, EE, and Starch on the Rumen Bacteria Community and Function in Dairy Cattle.IF=4.128

6.Yangyi Hao.2021.Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves.IF=2.752

7.Yujie Song.2022.Enzymes and microorganisms jointly promote the fermentation of rapeseed cake.IF=6.59

8.Jiaqi Wang.2023.l-Arginine treatment maintains postharvest quality in blueberry fruit by enhancing antioxidant capacity uring storage.IF=3.9

9.Juanjuan Chen.2023.Genome-Wide Detection of SPX Family and Profiling of CoSPX-MFS3 in Regulating Low-Phosphate Stress in Tea-Oil Camellia.IF=5.6

1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？

     答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。

          “24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。

2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？

     答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;

           微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。

3、问：分光法试剂盒与微板法试剂盒是否能通用？

     答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！