一、产品简介：
多酚氧化酶(polyphenol oxidase，EC 1.10.3.1，PPO)又称酪氨酸酶、儿茶酚酶、酚酶等，是自然界中分布极广的一种含铜氧化酶，普遍存在于植物、真菌、昆虫的质体中。植物受到机械损伤和病菌侵染后，PPO催化酚与O2氧化形成醌，使组织形成褐变．以便损伤恢复，防止或减少感染，提高抗病能力，与果蔬食品加工、储藏；茶叶品质和组培等密切相关。
多酚氧化酶PPO是一种含铜的氧化酶，能够催化邻苯二酚产生醌，后者在420nm有特征光吸收。
二、需自备的仪器和用品：
可见分光光度计、1mL玻璃比色皿（光径1cm）、台式离心机、水浴锅、可调式移液器、研钵、冰和蒸馏水。
1. Wei Gao. 2019. Design and Synthesis of N-phenyl Phthalimides as Potent Protoporphyrinogen Oxidase Inhibitors. Molecules. IF=4.4
2.Yi Gao.2022.Full-Scale of a Compost Process Using Swine Manure, Human Feces, and Rice Straw as Feedstock..IF=6.064
3.Yan Wang.2022.Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng.IF=6.064
4.Hubiao Jiang.2021.Effect of the Nanoparticle Exposures on the Tomato Bacterial Wilt Disease Control by Modulating the Rhizosphere Bacterial Community.IF=5.924
5.Yangyang Sun.2022.Phenylpropanoid metabolism in relation to peel browning development of cold-stored ‘Nanguo’ pears.IF=4.729
6.Liu, Xiaohui.2021.Study on browning mechanism of fresh-cut eggplant (Solanum melongena L.) based on metabolomics, enzymatic assays and gene expression.IF=4.38
7.Ting Fang.2022.Phytic Acid Treatment Inhibits Browning and Lignification to Promote the Quality of Fresh-Cut Apples during Storage.4.35
8.Yun-Ze Chen.2021.Transcriptomic analysis of interactions between Lymantria dispar larvae and carvacrol.IF=3.963
9.Wei Gao.2019.Design and Synthesis of N-phenyl Phthalimides as Potent Protoporphyrinogen Oxidase Inhibitors.IF=3.06
10.Xiao, Kai.2022.Genome-wide identification of polyphenol oxidase (PPO) family members in eggplant (Solanum melongena L.) and their expression in response to low temperature.IF=2.138
11.Caiyun Xiong.2022.Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred Lines.IF=6.208
12.Qin, Ruofan.2023.Analysis of oxidase activity and transcriptomic changes related to cutting propagation of hybrid larch.IF=4.996
13.Fulei Mo.2023.Genome-wide identification and expression analysis of SLAC1 gene family in tomato (Solanum lycopersicum) and the function of SlSLAC1–6 under cold stress.IF=4.342
14.Jinxin Liu.2023.Biocontrol ability and action mechanism of Bacillus amyloliquefaciens Baf1 against Fusarium incarnatum causing fruit rot in postharvest muskmelon (cv. Yugu) fruit.IF=6.056
15.Xiaoyan Wu.2023.Design and synthesis of novel PPO-inhibiting pyrimidinedione derivatives safed towards cotton.IF=4.966
16.Jiang Tangxin.Effects of thidiazuron on the quality and storage properties of mango fruit during postharvest.Food Quality and Safety.IF=5.6
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
