一、产品简介:
超氧化物歧化酶(SOD)(EC 1.15.1.1)在动植物、微生物和培养细胞体内广泛存在,其具有抗衰老、提高机体对多种疾病的抵抗力,能增强机体对外界环境的适应力。
本试剂盒是NBT法测定SOD活性,NBT可以和黄嘌呤氧化酶(Xanthine Oxidase, XO)催化产生的超氧化物阴离子(O2.- )反应产生有颜色物质,后者在560nm处有吸收;SOD可清除O2.-,从而抑制有色物质形成;反应液颜色越深,说明SOD活性愈低,反之活性越高。
二、所需的仪器和用品:
酶标仪、96孔板、低温离心机、可调式移液器、研钵、冰和蒸馏水
序号 | 论文标题 | 期刊 | IF | 原文链接 |
1 | Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different Durations | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/article/10.3390/ijms23179599 |
2 | Antifungal Activity of 6-Methylcoumarin against Valsa mali and Its Possible Mechanism of Action | Journal of Fungi | 5.724 | https://www.mdpi.com/2018212 |
3 | Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 8.025 | https://www.sciencedirect.com/science/article/pii/S0141813023002842 |
4 | Low temperature response index for monitoring freezing injury of tea plant | Frontiers in Plant Science | 6.627 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933980/ |
5 | Genome-wide characterization and expression of DELLA genes in Cucurbita moschata reveal their potential roles under development and abiotic stress | Frontiers in Plant Science | 6.627 | https://pubmed.ncbi.nlm.nih.gov/36909418/ |
6 | Mineral and Metabolome Analyses Provide Insights into the Cork Spot Disorder on ‘Akizuki’ Pear Fruit | Horticulturae | 3.1 | https://www.mdpi.com/2311-7524/9/7/818 |
7 | Analysis of N6-methyladenosine reveals a new important mechanism regulating the salt tolerance of sugar beet (Beta vulgaris) | PLANT SCIENCE | 5.2 | https://www.sciencedirect.com/science/article/pii/S016894522300211X |
8 | Unique Features of the m6A Methylome and Its Response to Salt Stress in the Roots of Sugar Beet (Beta vulgaris) | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 5.6 | https://www.mdpi.com/1422-0067/24/14/11659 |
1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?
答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。
2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?
答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;
微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。
3、问:分光法试剂盒与微板法试剂盒是否能通用?
答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!