一、产品简介:
过氧化物酶(POD,EC 1.11.1.7)广泛存在于动物、植物、微生物和培养细胞中, 是普遍存在的一种重要的氧化还原酶,其活性高低与抗性密切相关。在过氧化物酶催化 下,H2O2氧化愈创木酚生成红棕色产物,该产物在 470nm 处有最大光吸收,故可通过 测 470nm 下吸光值变化测定过氧化物酶活性。
二、需自备的仪器和用品:
酶标仪、96 孔板、台式离心机、可调式移液器、研钵、冰和蒸馏水。
序号 | 论文标题 | 期刊 | IF | 原文链接 |
1 | Polyethyleneimine-coated MXene quantum dots improve cotton tolerance to Verticillium dahliae by maintaining ROS homeostasis | Nature Communications | 16.6 | https://www.nature.com/articles/s41467-023-43192-4 |
2 | Concurrence of microplastics and heat waves reduces rice yields and disturbs the agroecosystem nitrogen cycle | JOURNAL OF HAZARDOUS MATERIALS | 14.224 | https://www.sciencedirect.com/science/article/pii/S0304389423006234 |
3 | Melatonin enhances KCl salinity tolerance by maintaining K+ homeostasis in Malus hupehensis | PLANT BIOTECHNOLOGY JOURNAL | 13.8 | https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.14129 |
4 | Quorum sensing signal autoinducer-2 promotes hydrogen peroxide degradation in water by Gram-positive bacteria | JOURNAL OF HAZARDOUS MATERIALS | 13.6 | https://www.sciencedirect.com/science/article/pii/S0304389424001614 |
5 | Integrative chemical, physiological, and metabolomics analyses reveal nanospecific phytotoxicity of metal nanoparticles | JOURNAL OF ENVIRONMENTAL MANAGEMENT | 8.7 | https://www.sciencedirect.com/science/article/pii/S0301479724003244 |
6 | Comparison of bio-beads combined with Pseudomonas edaphica and three phosphate materials for lead immobilization: Performance, mechanism and plant growth | JOURNAL OF ENVIRONMENTAL MANAGEMENT | 8.7 | https://www.sciencedirect.com/science/article/pii/S0301479724007837 |
7 | Enhancing malting performance of harder barley varieties through ultrasound treatment | ULTRASONICS SONOCHEMISTRY | 8.4 | https://www.sciencedirect.com/science/article/pii/S1350417724001081 |
8 | Synthesis of chitin nanocrystals supported Zn2+ with high activity against tobacco mosaic virus | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 8.2 | https://www.sciencedirect.com/science/article/pii/S0141813023030647 |
9 | Overexpression of MiSPL3a and MiSPL3b confers early flowering and stress tolerance in Arabidopsis thaliana | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 8.2 | https://www.sciencedirect.com/science/article/pii/S0141813024007165 |
10 | Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 8.025 | https://www.sciencedirect.com/science/article/pii/S0141813023002842 |
11 | Coupling raw material cultivation with nano-hydroxyapatite application to utilize and remediate severely Cd-containing soil | PROCESS SAFETY AND ENVIRONMENTAL PROTECTION | 7.8 | https://www.sciencedirect.com/science/article/pii/S0957582024001125 |
12 | Transcriptome and Metabolome Analyses Reveal Complex Molecular Mechanisms Involved in the Salt Tolerance of Rice Induced by Exogenous Allantoin | Antioxidants | 7.675 | https://www.mdpi.com/1890910 |
13 | A bamboo bHLH transcription factor PeRHL4 has dual functions in enhancing drought and phosphorus starvation tolerance | PLANT CELL AND ENVIRONMENT | 7.3 | https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.14920 |
14 | Cellobiose elicits immunity in lettuce conferring resistance against | JOURNAL OF EXPERIMENTAL BOTANY | 7.298 | https://academic.oup.com/jxb/advance-article-abstract/doi/10.1093/jxb/erac448/6831719 |
15 | CsAP2L transcription factor regulates resistance of citrus fruit to Penicillium digitatum through lignin biosynthesis and ROS metabolism pathways | POSTHARVEST BIOLOGY AND TECHNOLOGY | 7 | https://www.sciencedirect.com/science/article/pii/S0925521423002600 |
16 | Melatonin Alleviates Antimony Toxicity by Regulating the Antioxidant Response and Reducing Antimony Accumulation in Oryza sativa L. | Antioxidants | 7 | https://www.mdpi.com/2076-3921/12/11/1917 |
17 | ABC transporter SlABCG23 regulates chilling resistance of tomato fruit by affecting JA signaling pathway | POSTHARVEST BIOLOGY AND TECHNOLOGY | 7 | https://www.sciencedirect.com/science/article/pii/S0925521423004234 |
18 | Methionine enhances disease resistance of jujube fruit against postharvest black spot rot by activating lignin biosynthesis | POSTHARVEST BIOLOGY AND TECHNOLOGY | 6.751 | https://www.sciencedirect.com/science/article/pii/S092552142200103X |
19 | Methionine enhances disease resistance of jujube fruit against postharvest black spot rot by activating lignin biosynthesis | POSTHARVEST BIOLOGY AND TECHNOLOGY | 6.751 | https://www.sciencedirect.com/science/article/pii/S092552142200103X |
20 | Flavonoid accumulation modulates the responses of cassava tuberous roots to postharvest physiological deterioration | POSTHARVEST BIOLOGY AND TECHNOLOGY | 6.751 | https://www.sciencedirect.com/science/article/pii/S0925521423000157 |
21 | Transcription factor CsERF1B regulates postharvest citrus fruit resistance to Penicillium digitatum | POSTHARVEST BIOLOGY AND TECHNOLOGY | 6.751 | https://www.sciencedirect.com/science/article/pii/S0925521423000212 |
22 | The regulatory pathway of transcription factor MYB36 from Trichoderma asperellum Tas653 resistant to poplar leaf blight pathogen Alternaria alternata Aal004 | MICROBIOLOGICAL RESEARCH | 6.7 | https://www.sciencedirect.com/science/article/pii/S0944501324000387 |
23 | Structure, development, and the salt response of salt bladders in Chenopodium album L. | Frontiers in Plant Science | 6.627 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493005/ |
24 | Genome-wide analysis of the homeodomain-leucine zipper family in Lotus japonicus and the overexpression of LjHDZ7 in Arabidopsis for salt tolerance. | Frontiers in Plant Science | 6.627 | https://europepmc.org/articles/pmc9515785/bin/table_1.docx?javascript_support=no |
25 | Integrating GWAS and transcriptomics to identify candidate genes conferring heat tolerance in rice | Frontiers in Plant Science | 6.627 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868562/ |
26 | Genome-wide identification of the B3 gene family in soybean and the response to melatonin under cold stress | Frontiers in Plant Science | 6.627 | https://www.frontiersin.org/articles/10.3389/fpls.2022.1091907/full?amp;amp |
27 | Low temperature response index for monitoring freezing injury of tea plant | Frontiers in Plant Science | 6.627 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933980/ |
28 | Integration of transcriptome and metabolome analyses reveals sorghum roots responding to cadmium stress through regulation of the flavonoid biosynthesis pathway | Frontiers in Plant Science | 6.627 | https://pubmed.ncbi.nlm.nih.gov/36909379/ |
29 | Candidate genes conferring ethylene-response in cultivated peanuts determined by BSA-seq and fine-mapping | Crop Journal | 6.6 | https://www.sciencedirect.com/science/article/pii/S2214514124000576 |
30 | Metabolomics analysis of the nutraceutical diversity and physiological quality of Torreya yunnanensis seeds during cold storage | PLANT PHYSIOLOGY AND BIOCHEMISTRY | 6.5 | https://www.sciencedirect.com/science/article/pii/S0981942823006940 |
31 | LEAF TIP RUMPLED 1 Regulates Leaf Morphology and Salt Tolerance in Rice | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/article/10.3390/ijms23158818 |
32 | Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different Durations | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/article/10.3390/ijms23179599 |
33 | Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred Lines | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/1815860 |
34 | Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred Lines | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/1815860 |
35 | Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in Glycine max (L.) Merr. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/1887308 |
36 | Identification of KFB Family in Moso Bamboo Reveals the Potential Function of PeKFB9 Involved in Stress Response and Lignin Polymerization | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/1895178 |
37 | Cloning and Characterization of Two Novel PR4 Genes from Picea asperata | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/1974050 |
38 | GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.) | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/1422-0067/24/6/5547 |
39 | Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng | Frontiers in Microbiology | 6.064 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133625/ |
40 | Regulating Root Fungal Community Using Mortierella alpina for Fusarium oxysporum Resistance in Panax ginseng | Frontiers in Microbiology | 6.064 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133625/ |
41 | Synergic mitigation of saline-alkaline stress in wheat plant by silicon and Enterobacter sp. FN0603 | Frontiers in Microbiology | 6.064 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885204/ |
42 | Dark septate endophyte Exophiala pisciphila promotes maize growth and alleviates cadmium toxicity | Frontiers in Microbiology | 6.064 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126344/ |
43 | Biocontrol ability and action mechanism of Bacillus amyloliquefaciens Baf1 against Fusarium incarnatum causing fruit rot in postharvest muskmelon (cv. Yugu) fruit | LWT-FOOD SCIENCE AND TECHNOLOGY | 6.056 | https://www.sciencedirect.com/science/article/pii/S0023643823002931 |
44 | Sex-specific physiological and biochemical responses of Litsea cubeba under waterlogging stress | ENVIRONMENTAL AND EXPERIMENTAL BOTANY | 6.028 | https://www.sciencedirect.com/science/article/pii/S0098847222002404 |
45 | Sex-specific physiological and biochemical responses of Litsea cubeba under waterlogging stress | ENVIRONMENTAL AND EXPERIMENTAL BOTANY | 6.028 | https://www.sciencedirect.com/science/article/pii/S0098847222002404 |
46 | Effect of the Nanoparticle Exposures on the Tomato Bacterial Wilt Disease Control by Modulating the Rhizosphere Bacterial Community | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 5.924 | https://www.mdpi.com/1422-0067/23/1/414 |
47 | Effect of the Nanoparticle Exposures on the Tomato Bacterial Wilt Disease Control by Modulating the Rhizosphere Bacterial Community | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 5.924 | https://www.mdpi.com/1422-0067/23/1/414 |
48 | Integrated Physiological, Transcriptomic, and Metabolomic Analysis Reveals the Mechanism of Guvermectin Promoting Seed Germination in Direct-Seeded Rice under Chilling Stress | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 5.895 | https://pubs.acs.org/doi/abs/10.1021/acs.jafc.3c00559 |
49 | Resveratrol Alleviates the KCl Salinity Stress of Malus hupehensis Rhed | Frontiers in Plant Science | 5.754 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149799/ |
50 | Resveratrol Alleviates the KCl Salinity Stress of Malus hupehensis Rhed | Frontiers in Plant Science | 5.754 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149799/ |
51 | Integrated Analysis of Metabolome and Transcriptome Reveals Insights for Cold Tolerance in Rapeseed (Brassica napus L.) | Frontiers in Plant Science | 5.754 | https://www.frontiersin.org/articles/10.3389/fpls.2021.721681/full |
52 | Integrated Analysis of Metabolome and Transcriptome Reveals Insights for Cold Tolerance in Rapeseed (Brassica napus L.) | Frontiers in Plant Science | 5.754 | https://www.frontiersin.org/articles/10.3389/fpls.2021.721681/full |
53 | Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5 | Frontiers in Plant Science | 5.754 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649694/ |
54 | Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5 | Frontiers in Plant Science | 5.754 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649694/ |
55 | Mechanistic Insights Into Trehalose-Mediated Cold Stress Tolerance in Rapeseed ( Brassica napus L.) Seedlings | Frontiers in Plant Science | 5.754 | https://pubmed.ncbi.nlm.nih.gov/35360297/ |
56 | Mechanistic Insights Into Trehalose-Mediated Cold Stress Tolerance in Rapeseed ( Brassica napus L.) Seedlings | Frontiers in Plant Science | 5.754 | https://pubmed.ncbi.nlm.nih.gov/35360297/ |
57 | Importance of abscisic acid and zeatin nucleosides for the nitrate-induced cadmium hyperaccumulation in Populus roots | ENVIRONMENTAL AND EXPERIMENTAL BOTANY | 5.7 | https://www.sciencedirect.com/science/article/pii/S0098847224000728 |
58 | Unique Features of the m6A Methylome and Its Response to Salt Stress in the Roots of Sugar Beet (Beta vulgaris) | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 5.6 | https://www.mdpi.com/1422-0067/24/14/11659 |
59 | A microencapsulation approach to design microbial seed coatings to boost wheat seed germination and seedling growth under salt stress | Frontiers in Plant Science | 5.6 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10702945/ |
60 | Overexpression of a ‘Beta’ MYB Factor Gene, VhMYB15, Increases Salinity and Drought Tolerance in Arabidopsis thaliana | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 5.6 | https://www.mdpi.com/1422-0067/25/3/1534 |
61 | Whole-Transcriptome Profiling and Identification of Cold Tolerance-Related ceRNA Networks in Japonica Rice Varieties | Frontiers in Plant Science | 5.6 | https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1260591/abstract |
62 | Low-Toxicity Self-Photosensitized Biohybrid Systems for Enhanced Light-Driven H2 Production | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 5.6 | https://www.mdpi.com/1422-0067/25/6/3085 |
63 | ZmNAC17 Regulates Mesocotyl Elongation by Mediating Auxin and ROS Biosynthetic Pathways in Maize | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 5.6 | https://www.mdpi.com/1422-0067/25/9/4585 |
64 | The SsWRKY1 transcription factor of Saccharum spontaneum enhances drought tolerance in transgenic Arabidopsis thaliana and interacts with 21 potential proteins to regulate drought tolerance in S. spontaneum | PLANT PHYSIOLOGY AND BIOCHEMISTRY | 5.437 | https://www.sciencedirect.com/science/article/pii/S0981942823002176 |
65 | Responses of antioxidant enzymes and key resistant substances in perennial ryegrass (Lolium perenne L.) to cadmium and arsenic stresses | BMC PLANT BIOLOGY | 5.26 | https://link.springer.com/article/10.1186/s12870-022-03475-2 |
66 | Responses of antioxidant enzymes and key resistant substances in perennial ryegrass (Lolium perenne L.) to cadmium and arsenic stresses | BMC PLANT BIOLOGY | 5.26 | https://link.springer.com/article/10.1186/s12870-022-03475-2 |
67 | Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stage | FIELD CROPS RESEARCH | 5.224 | https://www.sciencedirect.com/science/article/pii/S0378429021003415 |
68 | Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stage | FIELD CROPS RESEARCH | 5.224 | https://www.sciencedirect.com/science/article/pii/S0378429021003415 |
69 | Analysis of N6-methyladenosine reveals a new important mechanism regulating the salt tolerance of sugar beet (Beta vulgaris) | PLANT SCIENCE | 5.2 | https://www.sciencedirect.com/science/article/pii/S016894522300211X |
70 | Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporum | Frontiers in Microbiology | 5.2 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623355/ |
71 | Sulfur fertiliser enhancement of Erigeron breviscapus (Asteraceae) quality by improving plant physiological responses and reducing soil cadmium bioavailability | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH | 5.19 | https://link.springer.com/article/10.1007/s11356-022-20778-x |
72 | Sulfur fertiliser enhancement of Erigeron breviscapus (Asteraceae) quality by improving plant physiological responses and reducing soil cadmium bioavailability | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH | 5.19 | https://link.springer.com/article/10.1007/s11356-022-20778-x |
73 | Role of reactive oxygen species (ROS) signaling pathway in the wound-induced spore formation of Pyropia yezoensis | Algal Research-Biomass Biofuels and Bioproducts | 5.1 | https://www.sciencedirect.com/science/article/pii/S2211926424000699 |
74 | Genetic transformation of LoHDZ2 and analysis of its function to enhance stress resistance in Larix olgensis | Scientific Reports | 4.996 | https://www.nature.com/articles/s41598-022-17191-2 |
75 | Genetic transformation of LoHDZ2 and analysis of its function to enhance stress resistance in Larix olgensis | Scientific Reports | 4.996 | https://www.nature.com/articles/s41598-022-17191-2 |
76 | Differential effects of low and high temperature stress on pollen germination and tube length of mango (Mangifera indica L.) genotypes | Scientific Reports | 4.996 | https://www.nature.com/articles/s41598-023-27917-5 |
77 | Analysis of oxidase activity and transcriptomic changes related to cutting propagation of hybrid larch | Scientific Reports | 4.996 | https://www.nature.com/articles/s41598-023-27779-x |
1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?
答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。
2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?
答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;
微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。
3、问:分光法试剂盒与微板法试剂盒是否能通用?
答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!