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超氧化物歧化酶(SOD)试剂盒-WST-8法

货号:G0102F
规格:96样
价格:720
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  • 产品简介
  • 已发文章
  • 产品问答


一、产品简介:

     超氧化物歧化酶(SOD)(EC 1.15.1.1)在动植物、微生物和培养细胞体内广泛存在, 其具有抗衰老、提高机体对多种疾病的抵抗力,能增强机体对外界环境的适应力,是生物体内一种重要的抗氧化酶。目前有多种 SOD 活性测定法,其中 NBT(氮蓝四唑)法产生的甲臜染料水溶性差,易和被还原的黄嘌呤氧化酶相互作用,抑制百分率达不到 100%等,从而使检测的灵敏度和精确度受到影响;本试剂盒采用的是目前稳定性更好、灵敏度更高的 WST-8 法,WST-8可以和黄嘌呤氧化酶(Xanthine Oxidase, XO)催化产生的超氧化物阴离子(O2.- )反应产生水溶性的甲臜染料,后者在 450nm 处有最大吸收;SOD 可清除 O2.-.,从而抑制甲臜的形成;反应液颜色越深,说明 SOD 活性愈低,反之活性越高。


二、所需的仪器和用品:

 可见分光光度计(波长:450nm)、1mL 玻璃比色皿(光径 1cm)、低温离心机、可调式移液器、研钵


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17.Yang Wang.2021.Protective Effect of Lactobacillus plantarum P8 on Growth Performance, Intestinal Health, and Microbiota in Eimeria-Infected Broilers.IF=5.64


18.Fuqi Wang.2021.Isorhamnetin, the xanthine oxidase inhibitor from Sophora japonica, ameliorates uric acid levels and renal function in hyperuricemic mice.IF=5.396


19.Jiang, Na.2022.Responses of antioxidant enzymes and key resistant substances in perennial ryegrass (Lolium perenne L.) to cadmium and arsenic stresses.IF=5.26


20.Yan Jia.2021.Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stage.IF=5.224


21.Liu, Yonglin.2022.Sulfur fertiliser enhancement of Erigeron breviscapus (Asteraceae) quality by improving plant physiological responses and reducing soil cadmium bioavailability.IF=5.19


22.An, Peiqi.2022.Genetic transformation of LoHDZ2 and analysis of its function to enhance stress resistance in Larix olgensis.IF=4.996


23.Sihong Liu.2020.Assessing the toxicity of three “inert” herbicide safeners toward Danio rerio: Effects on embryos development.IF=4.872


24.Shifa Xiong.2022.Effects of Drought Stress and Rehydration on Physiological and Biochemical Properties of Four Oak Species in China.IF=4.658


25.Liu, Xiaohui.2021.Study on browning mechanism of fresh-cut eggplant (Solanum melongena L.) based on metabolomics, enzymatic assays and gene expression.IF=4.38


26.Cheng Wang.2022.Hepatoprotective effect of phillygenin on carbon tetrachloride-induced liver fibrosis and its effects on short chain fatty acid and bile acid metabolism.IF=4.36


27.Xinyue Wei.2022.Some biochemical changes and transcriptome analysis associated with ‘Queen’ pineapple fruit blackheart development.IF=4.342


28.Hong Zhu.2021.The sweetpotato β-amylase gene IbBAM1.1 enhances drought and salt stress resistance by regulating ROS homeostasis and osmotic balance.IF=4.27


29.Wenwu Qiu.2020.Combined Analysis of Transcriptome and Metabolome Reveals the Potential Mechanism of Coloration and Fruit Quality in Yellow and Purple Passiflora edulis Sims.IF=4.192


30.Hong Zhu.2022.The Sweetpotato Voltage-Gated K+ Channel β Subunit, KIbB1, Positively Regulates Low-K+ and High-Salinity Tolerance by Maintaining Ion Homeostasis.IF=4.141


31.Xiaodong Zheng.2020.Exogenous Strigolactones alleviate KCl stress by regulating photosynthesis, ROS migration and ion transport in Malus hupehensis Rehd.IF=3.72


32.Jian Zhao.2022.Peptide OM-LV20 promotes structural and functional recovery of spinal cord injury in rats.IF=3.575


33.Li, Luhua.2022.Wheat TaANS-6D positively regulates leaf senescence through the abscisic acid mediated chlorophyll degradation in tobacco.IF=3.412


34.Chuang Zhang.2021.Vitexin ameliorates glycochenodeoxycholate-induced hepatocyte injury through SIRT6 and JAK2/STAT3 pathways.IF=2.699


35.Liu Yang.2020.Evaluating physiological changes of grass and semishrub species with seasonality for understanding the process of shrub encroachment in semiarid grasslands.IF=2.617


36.Chen, Siting.2022.Overexpression of the intertidal seagrass J protein ZjDjB1 enhances tolerance to chilling injury.IF=2.496


37.Li, Luhua.2022.Overexpression of TaLAX3-1B alters the stomatal aperture and improves the salt stress resistance of tobacco.IF=2.316


38.Xiao, Kai.2022.Genome-wide identification of polyphenol oxidase (PPO) family members in eggplant (Solanum melongena L.) and their expression in response to low temperature.IF=2.138


39.Caiyun Xiong.2022.Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred Lines.IF=6.208


40.Mengzhuo Zhang.2022.Physiological and Transcriptome Analyses of CaCl2 Treatment to Alleviate Chilling Injury in Pineapple.IF=4.658


41.Yigong Zhang.2022.Structure, development, and the salt response of salt bladders in Chenopodium album L..IF=6.627


42.Dan Wang.2022.Genome-wide analysis of the homeodomain-leucine zipper family in Lotus japonicus and the overexpression of LjHDZ7 in Arabidopsis for salt tolerance..IF=6.627


43.Yu-Xuan Wu.2022.Inhibitory effect and mechanism of action of juniper essential oil on gray mold in cherry tomatoes.IF=6.064


44.Chen, Siting.2022.Overexpression of Zostera japonica 14-3-3 gene ZjGRF1 enhances the resistance of transgenic Arabidopsis to copper stress.IF=2.742


45.Juan Wang.2022.Transcriptome and Metabolome Analyses Reveal Complex Molecular Mechanisms Involved in the Salt Tolerance of Rice Induced by Exogenous Allantoin.IF=7.675


46.Zhanyu Chen.2022.Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in Glycine max (L.) Merr.IF=6.208


47.Chen, Siting.2022.Overexpression of the intertidal seagrass 14-3-3 gene ZjGRF1 enhances the tolerance of transgenic Arabidopsis to salt and osmotic stress.IF=2.496


48.He Jiuxing.2022.Cellobiose elicits immunity in lettuce conferring resistance against.IF=7.298


49.Weidong Zhao.2022.Cloning and Characterization of Two Novel PR4 Genes from Picea asperata.IF=6.208


50.Feifei An.2023.Flavonoid accumulation modulates the responses of cassava tuberous roots to postharvest physiological deterioration.IF=6.751


51.Mengqi Zhang.2023.Effects of light on growth, feeding rate, digestion, and antioxidation in juvenile razor clams Sinonovacula constricta.IF=5.135


52.Liu, Xinyu.2023.Differential effects of low and high temperature stress on pollen germination and tube length of mango (Mangifera indica L.) genotypes.IF=4.996


53.Na Li.2023.Phytic acid is a new substitutable plant-derived antifungal agent for the seedling blight of Pinus sylvestris var. mongolica caused by Fusarium oxysporum..IF=4.966


54.Li, Yanmei.2023.VaSUS2 confers cold tolerance in transgenic tomato and Arabidopsis by regulation of sucrose metabolism and ROS homeostasis.IF=4.964


55.Hang  Yang.2023.Artemisia baimaensis allelopathy has a negative effect on the establishment of Elymus nutans artificial grassland in natural grassland.IF=2.734


56.Zhiyin Jiao.2023.Integration of transcriptome and metabolome analyses reveals sorghum roots responding to cadmium stress through regulation of the flavonoid biosynthesis pathway.IF=6.627


57.Fulei Mo.2023.Genome-wide identification and expression analysis of SLAC1 gene family in tomato (Solanum lycopersicum) and the function of SlSLAC1–6 under cold stress.IF=4.342


58.Xingang Li.2023.GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.).IF=6.208


59.Liu B. S.2023.Effects of Light Intensity on Morphological Structure and Physiological Characteristics of Gleditsia sinensis Seedlings.IF=1.419


60.Shuqing Guo.2023.Concurrence of microplastics and heat waves reduces rice yields and disturbs the agroecosystem nitrogen cycle.IF=14.224


61.Qing-Qing Shen.2023.The SsWRKY1 transcription factor of Saccharum spontaneum enhances drought tolerance in transgenic Arabidopsis thaliana and interacts with 21 potential proteins to regulate drought tolerance in S. spontaneum.IF=5.437


62.Xiaomei Li.2023.Characterization of Chlorophyll Fluorescence and Antioxidant Defense Parameters of Two Gracilariopsis lemaneiformis Strains under Different Temperatures.IF=4.658


63.Chen Siting.2023.Overexpression of Zostera japonica J protein gene ZjDjB1 in Arabidopsis enhanced the tolerance to lead stress.IF=2.742


64.Chongxi Liu.2023.Integrated Physiological, Transcriptomic, and Metabolomic Analysis Reveals the Mechanism of Guvermectin Promoting Seed Germination in Direct-Seeded Rice under Chilling Stress.IF=5.895


65.Hao Wu.2023.Disruption of LEAF LESION MIMIC 4 affects ABA synthesis and ROS accumulation in rice.IF=4.647


66.Cun Yu.2023.Trichoderma longibrachiatum Inoculation Improves Drought Resistance and Growth of Pinus massoniana Seedlings through Regulating Physiological Responses and Soil Microbial Community.IF=4.7


67.Hong Zhu.2023.The Sweet Potato K+ Transporter IbHAK11 Regulates K+ Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion Homeostasis.IF=4.5


68.Jia-jun Li.2023.Genome-wide identification of the mango pathogenesis-related 1 (PR1) gene family and functional analysis of MiPR1A genes in transgenic Arabidopsis.IF=4.3


69.Jia Shuao.2023.Appropriate carbon–nitrogen ratio is beneficial to the accumulation of 9-cis-β-carotene during Dunaliella salina cultivation.IF=3.3


70.Xu Simin.2023.Enhancing the Thermotolerance of Isochrysis zhangjiangensis Through Co-culturing With Algoriphagus marincola.IF=3


71.Zhijuan Sun.2023.Melatonin enhances KCl salinity tolerance by maintaining K+ homeostasis in Malus hupehensis.IF=13.8


72.Hao Chen.2023.Enhancing the Adaptability of Tea Plants (Camellia sinensis L.) to High-Temperature Stress with Small Peptides and Biosurfactants.IF=4.5


73.Baihui Jiang.Genome-wide identification of Glutathione peroxidase (GPX) family genes and silencing TaGPX3.2A reduced disease resistance in wheat.plant physiology and biochemistry.IF=6.5


74.Liu Jianguo.OsSTS, a Novel Allele of Mitogen-Activated Protein Kinase Kinase 4 (OsMKK4), Controls Grain Size and Salt Tolerance in Rice.Rice.IF=5.5


75.Lei Sun.Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporum.Frontiers in Microbiology.IF=5.2


76.Peng Mu.Genomic features of a plant growth-promoting endophytic Enterobacter cancerogenus JY65 dominant in microbiota of halophyte Suaeda salsa.IF=4.9


77.Yutan Chu.Melatonin Alleviates Antimony Toxicity by Regulating the Antioxidant Response and Reducing Antimony Accumulation in Oryza sativa L..Antioxidants.IF=7


78.Ye Yuan.Promotional Properties of ACC Deaminase-Producing Bacterial Strain DY1-3 and Its Enhancement of Maize Resistance to Salt and Drought Stresses.Microorganisms.IF=4.5








1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?

     答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。

          “24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。


2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?

     答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;

           微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。


3、问:分光法试剂盒与微板法试剂盒是否能通用?

     答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!