一、产品简介：
超氧化物歧化酶（SOD）（EC 1.15.1.1）在动植物、微生物和培养细胞体内广泛存在， 其具有抗衰老、提高机体对多种疾病的抵抗力，能增强机体对外界环境的适应力，是生物体内一种重要的抗氧化酶。目前有多种 SOD 活性测定法，其中 NBT(氮蓝四唑)法产生的甲臜染料水溶性差，易和被还原的黄嘌呤氧化酶相互作用，抑制百分率达不到 100%等，从而使检测的灵敏度和精确度受到影响；本试剂盒采用的是目前稳定性更好、灵敏度更高的 WST-8 法，WST-8可以和黄嘌呤氧化酶(Xanthine Oxidase, XO)催化产生的超氧化物阴离子(O2.- )反应产生水溶性的甲臜染料，后者在 450nm 处有最大吸收；SOD 可清除 O2.-.，从而抑制甲臜的形成；反应液颜色越深，说明 SOD 活性愈低，反之活性越高。
二、所需的仪器和用品：
酶标仪(波长：450nm)、96 孔板、低温离心机、可调式移液器、研钵、冰和蒸馏水。
| 序号 | 论文标题 | 期刊 | IF | 原文链接 |
| 1 | Evaluating physiological changes of grass and semishrub species with seasonality for understanding the process of shrub encroachment in semiarid grasslands | FUNCTIONAL PLANT BIOLOGY | 2.617 | https://www.publish.csiro.au/fp/fp19194 |
| 2 | Combined Analysis of Transcriptome and Metabolome Reveals the Potential Mechanism of Coloration and Fruit Quality in Yellow and Purple Passiflora edulis Sims | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 4.192 | https://pubs.acs.org/doi/abs/10.1021/acs.jafc.0c03619 |
| 3 | Developmental toxicity and transcriptome analysis of zebrafish (Danio rerio) embryos following exposure to chiral herbicide safener benoxacor | SCIENCE OF THE TOTAL ENVIRONMENT | 6.551 | https://www.sciencedirect.com/science/article/pii/S0048969720368042 |
| 4 | Assessing the toxicity of three “inert” herbicide safeners toward Danio rerio: Effects on embryos development | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 4.872 | https://www.sciencedirect.com/science/article/pii/S0147651320314135 |
| 5 | Exogenous Strigolactones alleviate KCl stress by regulating photosynthesis, ROS migration and ion transport in Malus hupehensis Rehd | PLANT PHYSIOLOGY AND BIOCHEMISTRY | 3.72 | https://www.sciencedirect.com/science/article/pii/S0981942820306380 |
| 6 | Protective Effect of Lactobacillus plantarum P8 on Growth Performance, Intestinal Health, and Microbiota in Eimeria-Infected Broilers | Frontiers in Microbiology | 5.64 | https://www.frontiersin.org/articles/10.3389/fmicb.2021.705758/full |
| 7 | Study on browning mechanism of fresh-cut eggplant (Solanum melongena L.) based on metabolomics, enzymatic assays and gene expression | Scientific Reports | 4.38 | https://www.nature.com/articles/s41598-021-86311-1 |
| 8 | Resveratrol Alleviates the KCl Salinity Stress of Malus hupehensis Rhed | Frontiers in Plant Science | 5.754 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149799/ |
| 9 | Protective Effect of Lactobacillus plantarum P8 on Growth Performance, Intestinal Health, and Microbiota in Eimeria-Infected Broilers | Frontiers in Microbiology | 5.64 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299996/ |
| 10 | The sweetpotato β-amylase gene IbBAM1.1 enhances drought and salt stress resistance by regulating ROS homeostasis and osmotic balance | PLANT PHYSIOLOGY AND BIOCHEMISTRY | 4.27 | https://www.sciencedirect.com/science/article/pii/S098194282100499X |
| 11 | Integrated Analysis of Metabolome and Transcriptome Reveals Insights for Cold Tolerance in Rapeseed (Brassica napus L.) | Frontiers in Plant Science | 5.754 | https://www.frontiersin.org/articles/10.3389/fpls.2021.721681/full |
| 12 | Isorhamnetin, the xanthine oxidase inhibitor from Sophora japonica, ameliorates uric acid levels and renal function in hyperuricemic mice | Food & Function | 5.396 | https://pubs.rsc.org/en/content/articlelanding/2021/fo/d1fo02719k |
| 13 | Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5 | Frontiers in Plant Science | 5.754 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649694/ |
| 14 | Vitexin ameliorates glycochenodeoxycholate-induced hepatocyte injury through SIRT6 and JAK2/STAT3 pathways | Iranian Journal of Basic Medical Sciences | 2.699 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976905/ |
| 15 | Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stage | FIELD CROPS RESEARCH | 5.224 | https://www.sciencedirect.com/science/article/pii/S0378429021003415 |
| 16 | Effect of the Nanoparticle Exposures on the Tomato Bacterial Wilt Disease Control by Modulating the Rhizosphere Bacterial Community | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 5.924 | https://www.mdpi.com/1422-0067/23/1/414 |
| 17 | Peptide OM-LV20 promotes structural and functional recovery of spinal cord injury in rats | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 3.575 | https://www.sciencedirect.com/science/article/pii/S0006291X22001991 |
| 18 | Mechanistic Insights into Stereospecific Antifungal Activity of Chiral Fungicide Prothioconazole against Fusarium oxysporum F. sp. cubense | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 5.924 | https://www.mdpi.com/1422-0067/23/4/2352 |
| 19 | Effects of Drought Stress and Rehydration on Physiological and Biochemical Properties of Four Oak Species in China | Plants-Basel | 4.658 | https://www.mdpi.com/2223-7747/11/5/679 |
| 20 | Mechanistic Insights Into Trehalose-Mediated Cold Stress Tolerance in Rapeseed ( Brassica napus L.) Seedlings | Frontiers in Plant Science | 5.754 | https://pubmed.ncbi.nlm.nih.gov/35360297/ |
| 21 | Responses of antioxidant enzymes and key resistant substances in perennial ryegrass (Lolium perenne L.) to cadmium and arsenic stresses | BMC PLANT BIOLOGY | 5.26 | https://link.springer.com/article/10.1186/s12870-022-03475-2 |
| 22 | Combined effects of S-metolachlor and benoxacor on embryo development in zebrafish (Danio rerio) | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 7.129 | https://www.sciencedirect.com/science/article/pii/S0147651322004055 |
| 23 | Sulfur fertiliser enhancement of Erigeron breviscapus (Asteraceae) quality by improving plant physiological responses and reducing soil cadmium bioavailability | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH | 5.19 | https://link.springer.com/article/10.1007/s11356-022-20778-x |
| 24 | Overexpression of the intertidal seagrass J protein ZjDjB1 enhances tolerance to chilling injury | Plant Biotechnology Reports | 2.496 | https://link.springer.com/article/10.1007/s11816-022-00761-1 |
| 25 | Overexpression of TaLAX3-1B alters the stomatal aperture and improves the salt stress resistance of tobacco | MOLECULAR BIOLOGY REPORTS | 2.316 | https://link.springer.com/article/10.1007/s11033-022-07548-1 |
| 26 | Wheat TaANS-6D positively regulates leaf senescence through the abscisic acid mediated chlorophyll degradation in tobacco | PLANT GROWTH REGULATION | 3.412 | https://link.springer.com/article/10.1007/s10725-022-00839-4 |
| 27 | Hepatoprotective effect of phillygenin on carbon tetrachloride-induced liver fibrosis and its effects on short chain fatty acid and bile acid metabolism | JOURNAL OF ETHNOPHARMACOLOGY | 4.36 | https://www.sciencedirect.com/science/article/pii/S0378874122005177 |
| 28 | The Sweetpotato Voltage-Gated K+ Channel β Subunit, KIbB1, Positively Regulates Low-K+ and High-Salinity Tolerance by Maintaining Ion Homeostasis | Genes | 4.141 | https://www.mdpi.com/article/10.3390/genes13061100 |
| 29 | Effects of Stocking Density on the Growth Performance, Physiological Parameters, Redox Status and Lipid Metabolism of Micropterus salmoides in Integrated Rice–Fish Farming Systems | Antioxidants | 7.675 | https://www.mdpi.com/2076-3921/11/7/1215 |
| 30 | Some biochemical changes and transcriptome analysis associated with ‘Queen’ pineapple fruit blackheart development | SCIENTIA HORTICULTURAE | 4.342 | https://www.sciencedirect.com/science/article/pii/S0304423822004101 |
| 31 | Genome-wide identification of polyphenol oxidase (PPO) family members in eggplant (Solanum melongena L.) and their expression in response to low temperature | Horticulture Environment and Biotechnology | 2.138 | https://link.springer.com/article/10.1007/s13580-022-00441-7 |
| 32 | Sex-specific physiological and biochemical responses of Litsea cubeba under waterlogging stress | ENVIRONMENTAL AND EXPERIMENTAL BOTANY | 6.028 | https://www.sciencedirect.com/science/article/pii/S0098847222002404 |
| 33 | Genetic transformation of LoHDZ2 and analysis of its function to enhance stress resistance in Larix olgensis | Scientific Reports | 4.996 | https://www.nature.com/articles/s41598-022-17191-2 |
| 34 | Physiological and Transcriptome Analyses of CaCl2 Treatment to Alleviate Chilling Injury in Pineapple | Plants-Basel | 4.658 | https://www.mdpi.com/1796736 |
| 35 | Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred Lines | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/1815860 |
| 36 | Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred Lines | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/1815860 |
| 37 | Structure, development, and the salt response of salt bladders in Chenopodium album L. | Frontiers in Plant Science | 6.627 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493005/ |
| 38 | Genome-wide analysis of the homeodomain-leucine zipper family in <i>Lotus japonicus</i> and the overexpression of <i>LjHDZ7</i> in Arabidopsis for salt tolerance. | Frontiers in Plant Science | 6.627 | https://europepmc.org/articles/pmc9515785/bin/table_1.docx?javascript_support=no |
| 39 | Inhibitory effect and mechanism of action of juniper essential oil on gray mold in cherry tomatoes | Frontiers in Microbiology | 6.064 | https://pubmed.ncbi.nlm.nih.gov/36212845/ |
| 40 | Overexpression of Zostera japonica 14-3-3 gene ZjGRF1 enhances the resistance of transgenic Arabidopsis to copper stress | MOLECULAR BIOLOGY REPORTS | 2.742 | https://link.springer.com/article/10.1007/s11033-022-07915-y |
| 41 | Overexpression of the intertidal seagrass 14-3-3 gene ZjGRF1 enhances the tolerance of transgenic Arabidopsis to salt and osmotic stress | Plant Biotechnology Reports | 2.496 | https://link.springer.com/article/10.1007/s11816-022-00795-5 |
| 42 | Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in Glycine max (L.) Merr. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/1887308 |
| 43 | Transcriptome and Metabolome Analyses Reveal Complex Molecular Mechanisms Involved in the Salt Tolerance of Rice Induced by Exogenous Allantoin | Antioxidants | 7.675 | https://www.mdpi.com/1890910 |
| 44 | Cellobiose elicits immunity in lettuce conferring resistance against | JOURNAL OF EXPERIMENTAL BOTANY | 7.298 | https://academic.oup.com/jxb/advance-article-abstract/doi/10.1093/jxb/erac448/6831719 |
| 45 | Cloning and Characterization of Two Novel PR4 Genes from Picea asperata | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/1974050 |
| 46 | Integrating GWAS and transcriptomics to identify candidate genes conferring heat tolerance in rice | Frontiers in Plant Science | 6.627 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868562/ |
| 47 | Flavonoid accumulation modulates the responses of cassava tuberous roots to postharvest physiological deterioration | POSTHARVEST BIOLOGY AND TECHNOLOGY | 6.751 | https://www.sciencedirect.com/science/article/pii/S0925521423000157 |
| 48 | Differential effects of low and high temperature stress on pollen germination and tube length of mango (Mangifera indica L.) genotypes | Scientific Reports | 4.996 | https://www.nature.com/articles/s41598-023-27917-5 |
| 49 | Effect of yeast culture supplementation in sows during late gestation and lactation on growth performance, antioxidant properties, and intestinal microorganisms of offspring weaned piglets | Frontiers in Microbiology | 6.064 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9880171/ |
| 50 | Phytic acid is a new substitutable plant-derived antifungal agent for the seedling blight of Pinus sylvestris var. mongolica caused by Fusarium oxysporum | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | 4.966 | https://www.sciencedirect.com/science/article/pii/S0048357523000068 |
| 51 | VaSUS2 confers cold tolerance in transgenic tomato and Arabidopsis by regulation of sucrose metabolism and ROS homeostasis | PLANT CELL REPORTS | 4.964 | https://link.springer.com/article/10.1007/s00299-022-02972-w |
| 52 | Artemisia baimaensis allelopathy has a negative effect on the establishment of Elymus nutans artificial grassland in natural grassland | Plant Signaling & Behavior | 2.734 | https://www.tandfonline.com/doi/abs/10.1080/15592324.2022.2163349 |
| 53 | Synergic mitigation of saline-alkaline stress in wheat plant by silicon and Enterobacter sp. FN0603 | Frontiers in Microbiology | 6.064 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885204/ |
| 54 | Effects of light on growth, feeding rate, digestion, and antioxidation in juvenile razor clams Sinonovacula constricta | AQUACULTURE | 5.135 | https://www.sciencedirect.com/science/article/pii/S0044848623000790 |
| 55 | Genome-wide identification and expression analysis of SLAC1 gene family in tomato (Solanum lycopersicum) and the function of SlSLAC1–6 under cold stress | SCIENTIA HORTICULTURAE | 4.342 | https://www.sciencedirect.com/science/article/pii/S0304423823000791 |
| 56 | Integration of transcriptome and metabolome analyses reveals sorghum roots responding to cadmium stress through regulation of the flavonoid biosynthesis pathway | Frontiers in Plant Science | 6.627 | https://pubmed.ncbi.nlm.nih.gov/36909379/ |
| 57 | GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.) | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 6.208 | https://www.mdpi.com/1422-0067/24/6/5547 |
| 58 | Effects of Light Intensity on Morphological Structure and Physiological Characteristics of Gleditsia sinensis Seedlings | RUSSIAN JOURNAL OF PLANT PHYSIOLOGY | 1.419 | https://link.springer.com/article/10.1134/S1021443722602506 |
| 59 | Concurrence of microplastics and heat waves reduces rice yields and disturbs the agroecosystem nitrogen cycle | JOURNAL OF HAZARDOUS MATERIALS | 14.224 | https://www.sciencedirect.com/science/article/pii/S0304389423006234 |
| 60 | The SsWRKY1 transcription factor of Saccharum spontaneum enhances drought tolerance in transgenic Arabidopsis thaliana and interacts with 21 potential proteins to regulate drought tolerance in S. spontaneum | PLANT PHYSIOLOGY AND BIOCHEMISTRY | 5.437 | https://www.sciencedirect.com/science/article/pii/S0981942823002176 |
| 61 | Characterization of Chlorophyll Fluorescence and Antioxidant Defense Parameters of Two Gracilariopsis lemaneiformis Strains under Different Temperatures | Plants-Basel | 4.658 | https://www.mdpi.com/2223-7747/12/8/1670 |
| 62 | Overexpression of Zostera japonica J protein gene ZjDjB1 in Arabidopsis enhanced the tolerance to lead stress | MOLECULAR BIOLOGY REPORTS | 2.742 | https://link.springer.com/article/10.1007/s11033-023-08470-w |
| 63 | Integrated Physiological, Transcriptomic, and Metabolomic Analysis Reveals the Mechanism of Guvermectin Promoting Seed Germination in Direct-Seeded Rice under Chilling Stress | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 5.895 | https://pubs.acs.org/doi/abs/10.1021/acs.jafc.3c00559 |
| 64 | Disruption of LEAF LESION MIMIC 4 affects ABA synthesis and ROS accumulation in rice | Crop Journal | 4.647 | https://www.sciencedirect.com/science/article/pii/S2214514123000491 |
| 65 | Enhancing the Thermotolerance of Isochrysis zhangjiangensis Through Co-culturing With Algoriphagus marincola | MARINE BIOTECHNOLOGY | 3 | https://link.springer.com/article/10.1007/s10126-023-10219-2 |
| 66 | Appropriate carbon–nitrogen ratio is beneficial to the accumulation of 9-cis-β-carotene during Dunaliella salina cultivation | JOURNAL OF APPLIED PHYCOLOGY | 3.3 | https://link.springer.com/article/10.1007/s10811-023-03008-w |
| 67 | Trichoderma longibrachiatum Inoculation Improves Drought Resistance and Growth of Pinus massoniana Seedlings through Regulating Physiological Responses and Soil Microbial Community | Journal of Fungi | 4.7 | https://www.mdpi.com/2309-608X/9/7/694 |
| 68 | The Sweet Potato K+ Transporter IbHAK11 Regulates K+ Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion Homeostasis | Plants-Basel | 4.5 | https://www.mdpi.com/2223-7747/12/13/2422 |
| 69 | Genome-wide identification of the mango pathogenesis-related 1 (PR1) gene family and functional analysis of MiPR1A genes in transgenic Arabidopsis | SCIENTIA HORTICULTURAE | 4.3 | https://www.sciencedirect.com/science/article/pii/S0304423823004259 |
| 70 | Melatonin enhances KCl salinity tolerance by maintaining K+ homeostasis in Malus hupehensis | PLANT BIOTECHNOLOGY JOURNAL | 13.8 | https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.14129 |
| 71 | Enhancing the Adaptability of Tea Plants (Camellia sinensis L.) to High-Temperature Stress with Small Peptides and Biosurfactants | Plants-Basel | 4.5 | https://www.mdpi.com/2223-7747/12/15/2817 |
| 72 | Synthesis of chitin nanocrystals supported Zn2+ with high activity against tobacco mosaic virus | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 8.2 | https://www.sciencedirect.com/science/article/pii/S0141813023030647 |
| 73 | Transcriptome and WGCNA reveal hub genes in sugarcane tiller seedlings in response to drought stress | Scientific Reports | 4.6 | https://www.nature.com/articles/s41598-023-40006-x |
| 74 | Transcription Factor GmERF105 Negatively Regulates Salt Stress Tolerance in Arabidopsis thaliana | Plants-Basel | 4.5 | https://www.mdpi.com/2223-7747/12/16/3007 |
| 75 | Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks | Antibiotics-Basel | 4.8 | https://www.mdpi.com/2079-6382/12/9/1356 |
| 76 | Genome-Wide and 16S rRNA Sequencing-Based Analysis on the Health Effects of Lacticaseibacillus paracasei XLK401 on Chicks | Microorganisms | 4.5 | https://www.mdpi.com/2076-2607/11/9/2140 |
| 77 | Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporum | Frontiers in Microbiology | 5.2 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623355/ |
| 78 | Genome-wide identification of Glutathione peroxidase (GPX) family genes and silencing TaGPX3.2A reduced disease resistance in wheat | PLANT PHYSIOLOGY AND BIOCHEMISTRY | 6.5 | https://www.sciencedirect.com/science/article/pii/S0981942823006502 |
| 79 | OsSTS, a Novel Allele of Mitogen-Activated Protein Kinase Kinase 4 (OsMKK4), Controls Grain Size and Salt Tolerance in Rice | Rice | 5.5 | https://thericejournal.springeropen.com/articles/10.1186/s12284-023-00663-y |
| 80 | Melatonin Alleviates Antimony Toxicity by Regulating the Antioxidant Response and Reducing Antimony Accumulation in Oryza sativa L. | Antioxidants | 7 | https://www.mdpi.com/2076-3921/12/11/1917 |
| 81 | Genomic features of a plant growth-promoting endophytic Enterobacter cancerogenus JY65 dominant in microbiota of halophyte Suaeda salsa | PLANT AND SOIL | 4.9 | https://link.springer.com/article/10.1007/s11104-023-06360-5 |
| 82 | Promotional Properties of ACC Deaminase-Producing Bacterial Strain DY1-3 and Its Enhancement of Maize Resistance to Salt and Drought Stresses | Microorganisms | 4.5 | https://www.mdpi.com/2076-2607/11/11/2654 |
| 83 | Integrating transcriptome and physiological analyses to elucidate the molecular responses of sorghum to fluxofenim and metolachlor herbicide | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | 4.7 | https://www.sciencedirect.com/science/article/pii/S0048357523003577 |
| 84 |
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？ 答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。 “24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。 2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？ 答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm; 微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。 3、问：分光法试剂盒与微板法试剂盒是否能通用？ 答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！ |
