0512-66188056

产品中心

技术服务 试剂盒开发

超氧化物歧化酶(SOD)试剂盒-WST-8法

货号:G0101F
规格:48样
价格:390
说明书:点击下载
提示:官网说明书如与试剂盒内纸质版有出入,请以纸质版为准!

  • 产品简介
  • 已发文章
  • 产品问答


一、产品简介:

     超氧化物歧化酶(SOD)(EC 1.15.1.1)在动植物、微生物和培养细胞体内广泛存在, 其具有抗衰老、提高机体对多种疾病的抵抗力,能增强机体对外界环境的适应力,是生物体内一种重要的抗氧化酶。目前有多种 SOD 活性测定法,其中 NBT(氮蓝四唑)法产生的甲臜染料水溶性差,易和被还原的黄嘌呤氧化酶相互作用,抑制百分率达不到 100%等,从而使检测的灵敏度和精确度受到影响;本试剂盒采用的是目前稳定性更好、灵敏度更高的 WST-8 法,WST-8可以和黄嘌呤氧化酶(Xanthine Oxidase, XO)催化产生的超氧化物阴离子(O2.- )反应产生水溶性的甲臜染料,后者在 450nm 处有最大吸收;SOD 可清除 O2.-.,从而抑制甲臜的形成;反应液颜色越深,说明 SOD 活性愈低,反之活性越高。


二、所需的仪器和用品:

 可见分光光度计(波长:450nm)、1mL 玻璃比色皿(光径 1cm)、低温离心机、可调式移液器、研钵




序号论文标题期刊IF原文链接
1Evaluating physiological changes of grass and semishrub species with seasonality for understanding the process of shrub encroachment in semiarid grasslandsFUNCTIONAL PLANT BIOLOGY2.617https://www.publish.csiro.au/fp/fp19194
2Combined Analysis of Transcriptome and Metabolome Reveals the Potential Mechanism of Coloration and Fruit Quality in Yellow and Purple Passiflora edulis SimsJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY4.192https://pubs.acs.org/doi/abs/10.1021/acs.jafc.0c03619
3Developmental toxicity and transcriptome analysis of zebrafish (Danio rerio) embryos following exposure to chiral herbicide safener benoxacorSCIENCE OF THE TOTAL ENVIRONMENT6.551https://www.sciencedirect.com/science/article/pii/S0048969720368042
4Assessing the toxicity of three “inert” herbicide safeners toward Danio rerio: Effects on embryos developmentECOTOXICOLOGY AND ENVIRONMENTAL SAFETY4.872https://www.sciencedirect.com/science/article/pii/S0147651320314135
5Exogenous Strigolactones alleviate KCl stress by regulating photosynthesis, ROS migration and ion transport in Malus hupehensis RehdPLANT PHYSIOLOGY AND BIOCHEMISTRY3.72https://www.sciencedirect.com/science/article/pii/S0981942820306380
6Protective Effect of Lactobacillus plantarum P8 on Growth Performance, Intestinal Health, and Microbiota in Eimeria-Infected BroilersFrontiers in Microbiology5.64https://www.frontiersin.org/articles/10.3389/fmicb.2021.705758/full
7Study on browning mechanism of fresh-cut eggplant (Solanum melongena L.) based on metabolomics, enzymatic assays and gene expressionScientific Reports4.38https://www.nature.com/articles/s41598-021-86311-1
8Resveratrol Alleviates the KCl Salinity Stress of Malus hupehensis RhedFrontiers in Plant Science5.754https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149799/
9Protective Effect of Lactobacillus plantarum P8 on Growth Performance, Intestinal Health, and Microbiota in Eimeria-Infected BroilersFrontiers in Microbiology5.64https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299996/
10The sweetpotato β-amylase gene IbBAM1.1 enhances drought and salt stress resistance by regulating ROS homeostasis and osmotic balancePLANT PHYSIOLOGY AND BIOCHEMISTRY4.27https://www.sciencedirect.com/science/article/pii/S098194282100499X
11Integrated Analysis of Metabolome and Transcriptome Reveals Insights for Cold Tolerance in Rapeseed (Brassica napus L.)Frontiers in Plant Science5.754https://www.frontiersin.org/articles/10.3389/fpls.2021.721681/full
12Isorhamnetin, the xanthine oxidase inhibitor from Sophora japonica, ameliorates uric acid levels and renal function in hyperuricemic miceFood & Function5.396https://pubs.rsc.org/en/content/articlelanding/2021/fo/d1fo02719k
13Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5Frontiers in Plant Science5.754https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649694/
14Vitexin ameliorates glycochenodeoxycholate-induced hepatocyte injury through SIRT6 and JAK2/STAT3 pathwaysIranian Journal of Basic Medical Sciences2.699https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976905/
15Effects of root characteristics on panicle formation in japonica rice under low temperature water stress at the reproductive stageFIELD CROPS RESEARCH5.224https://www.sciencedirect.com/science/article/pii/S0378429021003415
16Effect of the Nanoparticle Exposures on the Tomato Bacterial Wilt Disease Control by Modulating the Rhizosphere Bacterial CommunityINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES5.924https://www.mdpi.com/1422-0067/23/1/414
17Peptide OM-LV20 promotes structural and functional recovery of spinal cord injury in ratsBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS3.575https://www.sciencedirect.com/science/article/pii/S0006291X22001991
18Mechanistic Insights into Stereospecific Antifungal Activity of Chiral Fungicide Prothioconazole against Fusarium oxysporum F. sp. cubenseINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES5.924https://www.mdpi.com/1422-0067/23/4/2352
19Effects of Drought Stress and Rehydration on Physiological and Biochemical Properties of Four Oak Species in ChinaPlants-Basel4.658https://www.mdpi.com/2223-7747/11/5/679
20Mechanistic Insights Into Trehalose-Mediated Cold Stress Tolerance in Rapeseed ( Brassica napus L.) SeedlingsFrontiers in Plant Science5.754https://pubmed.ncbi.nlm.nih.gov/35360297/
21Responses of antioxidant enzymes and key resistant substances in perennial ryegrass (Lolium perenne L.) to cadmium and arsenic stressesBMC PLANT BIOLOGY5.26https://link.springer.com/article/10.1186/s12870-022-03475-2
22Combined effects of S-metolachlor and benoxacor on embryo development in zebrafish (Danio rerio)ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY7.129https://www.sciencedirect.com/science/article/pii/S0147651322004055
23Sulfur fertiliser enhancement of Erigeron breviscapus (Asteraceae) quality by improving plant physiological responses and reducing soil cadmium bioavailabilityENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH5.19https://link.springer.com/article/10.1007/s11356-022-20778-x
24Overexpression of the intertidal seagrass J protein ZjDjB1 enhances tolerance to chilling injuryPlant Biotechnology Reports2.496https://link.springer.com/article/10.1007/s11816-022-00761-1
25Overexpression of TaLAX3-1B alters the stomatal aperture and improves the salt stress resistance of tobaccoMOLECULAR BIOLOGY REPORTS2.316https://link.springer.com/article/10.1007/s11033-022-07548-1
26Wheat TaANS-6D positively regulates leaf senescence through the abscisic acid mediated chlorophyll degradation in tobaccoPLANT GROWTH REGULATION3.412https://link.springer.com/article/10.1007/s10725-022-00839-4
27Hepatoprotective effect of phillygenin on carbon tetrachloride-induced liver fibrosis and its effects on short chain fatty acid and bile acid metabolismJOURNAL OF ETHNOPHARMACOLOGY4.36https://www.sciencedirect.com/science/article/pii/S0378874122005177
28The Sweetpotato Voltage-Gated K+ Channel β Subunit, KIbB1, Positively Regulates Low-K+ and High-Salinity Tolerance by Maintaining Ion HomeostasisGenes4.141https://www.mdpi.com/article/10.3390/genes13061100
29Effects of Stocking Density on the Growth Performance, Physiological Parameters, Redox Status and Lipid Metabolism of Micropterus salmoides in Integrated Rice–Fish Farming SystemsAntioxidants7.675https://www.mdpi.com/2076-3921/11/7/1215
30Some biochemical changes and transcriptome analysis associated with ‘Queen’ pineapple fruit blackheart developmentSCIENTIA HORTICULTURAE4.342https://www.sciencedirect.com/science/article/pii/S0304423822004101
31Genome-wide identification of polyphenol oxidase (PPO) family members in eggplant (Solanum melongena L.) and their expression in response to low temperatureHorticulture Environment and Biotechnology2.138https://link.springer.com/article/10.1007/s13580-022-00441-7
32Sex-specific physiological and biochemical responses of Litsea cubeba under waterlogging stressENVIRONMENTAL AND EXPERIMENTAL BOTANY6.028https://www.sciencedirect.com/science/article/pii/S0098847222002404
33Genetic transformation of LoHDZ2 and analysis of its function to enhance stress resistance in Larix olgensisScientific Reports4.996https://www.nature.com/articles/s41598-022-17191-2
34Physiological and Transcriptome Analyses of CaCl2 Treatment to Alleviate Chilling Injury in PineapplePlants-Basel4.658https://www.mdpi.com/1796736
35Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred LinesINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES6.208https://www.mdpi.com/1815860
36Physiological and Molecular Characteristics of Southern Leaf Blight Resistance in Sweet Corn Inbred LinesINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES6.208https://www.mdpi.com/1815860
37Structure, development, and the salt response of salt bladders in Chenopodium album L.Frontiers in Plant Science6.627https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493005/
38Genome-wide analysis of the homeodomain-leucine zipper family in Lotus japonicus and the overexpression of LjHDZ7 in Arabidopsis for salt tolerance.Frontiers in Plant Science6.627https://europepmc.org/articles/pmc9515785/bin/table_1.docx?javascript_support=no
39Inhibitory effect and mechanism of action of juniper essential oil on gray mold in cherry tomatoesFrontiers in Microbiology6.064https://pubmed.ncbi.nlm.nih.gov/36212845/
40Overexpression of Zostera japonica 14-3-3 gene ZjGRF1 enhances the resistance of transgenic Arabidopsis to copper stressMOLECULAR BIOLOGY REPORTS2.742https://link.springer.com/article/10.1007/s11033-022-07915-y
41Overexpression of the intertidal seagrass 14-3-3 gene ZjGRF1 enhances the tolerance of transgenic Arabidopsis to salt and osmotic stressPlant Biotechnology Reports2.496https://link.springer.com/article/10.1007/s11816-022-00795-5
42Molecular Characterization and Drought Resistance of GmNAC3 Transcription Factor in Glycine max (L.) Merr.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES6.208https://www.mdpi.com/1887308
43Transcriptome and Metabolome Analyses Reveal Complex Molecular Mechanisms Involved in the Salt Tolerance of Rice Induced by Exogenous AllantoinAntioxidants7.675https://www.mdpi.com/1890910
44Cellobiose elicits immunity in lettuce conferring resistance againstJOURNAL OF EXPERIMENTAL BOTANY7.298https://academic.oup.com/jxb/advance-article-abstract/doi/10.1093/jxb/erac448/6831719
45Cloning and Characterization of Two Novel PR4 Genes from Picea asperataINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES6.208https://www.mdpi.com/1974050
46Integrating GWAS and transcriptomics to identify candidate genes conferring heat tolerance in riceFrontiers in Plant Science6.627https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868562/
47Flavonoid accumulation modulates the responses of cassava tuberous roots to postharvest physiological deteriorationPOSTHARVEST BIOLOGY AND TECHNOLOGY6.751https://www.sciencedirect.com/science/article/pii/S0925521423000157
48Differential effects of low and high temperature stress on pollen germination and tube length of mango (Mangifera indica L.) genotypesScientific Reports4.996https://www.nature.com/articles/s41598-023-27917-5
49Effect of yeast culture supplementation in sows during late gestation and lactation on growth performance, antioxidant properties, and intestinal microorganisms of offspring weaned pigletsFrontiers in Microbiology6.064https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9880171/
50Phytic acid is a new substitutable plant-derived antifungal agent for the seedling blight of Pinus sylvestris var. mongolica caused by Fusarium oxysporumPESTICIDE BIOCHEMISTRY AND PHYSIOLOGY4.966https://www.sciencedirect.com/science/article/pii/S0048357523000068
51VaSUS2 confers cold tolerance in transgenic tomato and Arabidopsis by regulation of sucrose metabolism and ROS homeostasisPLANT CELL REPORTS4.964https://link.springer.com/article/10.1007/s00299-022-02972-w
52Artemisia baimaensis allelopathy has a negative effect on the establishment of Elymus nutans artificial grassland in natural grasslandPlant Signaling & Behavior2.734https://www.tandfonline.com/doi/abs/10.1080/15592324.2022.2163349
53Synergic mitigation of saline-alkaline stress in wheat plant by silicon and Enterobacter sp. FN0603Frontiers in Microbiology6.064https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885204/
54Effects of light on growth, feeding rate, digestion, and antioxidation in juvenile razor clams Sinonovacula constrictaAQUACULTURE5.135https://www.sciencedirect.com/science/article/pii/S0044848623000790
55Genome-wide identification and expression analysis of SLAC1 gene family in tomato (Solanum lycopersicum) and the function of SlSLAC1–6 under cold stressSCIENTIA HORTICULTURAE4.342https://www.sciencedirect.com/science/article/pii/S0304423823000791
56Integration of transcriptome and metabolome analyses reveals sorghum roots responding to cadmium stress through regulation of the flavonoid biosynthesis pathwayFrontiers in Plant Science6.627https://pubmed.ncbi.nlm.nih.gov/36909379/
57GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.)INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES6.208https://www.mdpi.com/1422-0067/24/6/5547
58Effects of Light Intensity on Morphological Structure and Physiological Characteristics of Gleditsia sinensis SeedlingsRUSSIAN JOURNAL OF PLANT PHYSIOLOGY1.419https://link.springer.com/article/10.1134/S1021443722602506
59Concurrence of microplastics and heat waves reduces rice yields and disturbs the agroecosystem nitrogen cycleJOURNAL OF HAZARDOUS MATERIALS14.224https://www.sciencedirect.com/science/article/pii/S0304389423006234
60The SsWRKY1 transcription factor of Saccharum spontaneum enhances drought tolerance in transgenic Arabidopsis thaliana and interacts with 21 potential proteins to regulate drought tolerance in S. spontaneumPLANT PHYSIOLOGY AND BIOCHEMISTRY5.437https://www.sciencedirect.com/science/article/pii/S0981942823002176
61Characterization of Chlorophyll Fluorescence and Antioxidant Defense Parameters of Two Gracilariopsis lemaneiformis Strains under Different TemperaturesPlants-Basel4.658https://www.mdpi.com/2223-7747/12/8/1670
62Overexpression of Zostera japonica J protein gene ZjDjB1 in Arabidopsis enhanced the tolerance to lead stressMOLECULAR BIOLOGY REPORTS2.742https://link.springer.com/article/10.1007/s11033-023-08470-w
63Integrated Physiological, Transcriptomic, and Metabolomic Analysis Reveals the Mechanism of Guvermectin Promoting Seed Germination in Direct-Seeded Rice under Chilling StressJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY5.895https://pubs.acs.org/doi/abs/10.1021/acs.jafc.3c00559
64Disruption of LEAF LESION MIMIC 4 affects ABA synthesis and ROS accumulation in riceCrop Journal4.647https://www.sciencedirect.com/science/article/pii/S2214514123000491
65Enhancing the Thermotolerance of Isochrysis zhangjiangensis Through Co-culturing With Algoriphagus marincolaMARINE BIOTECHNOLOGY3https://link.springer.com/article/10.1007/s10126-023-10219-2
66Appropriate carbon–nitrogen ratio is beneficial to the accumulation of 9-cis-β-carotene during Dunaliella salina cultivationJOURNAL OF APPLIED PHYCOLOGY3.3https://link.springer.com/article/10.1007/s10811-023-03008-w
67Trichoderma longibrachiatum Inoculation Improves Drought Resistance and Growth of Pinus massoniana Seedlings through Regulating Physiological Responses and Soil Microbial CommunityJournal of Fungi4.7https://www.mdpi.com/2309-608X/9/7/694
68The Sweet Potato K+ Transporter IbHAK11 Regulates K+ Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion HomeostasisPlants-Basel4.5https://www.mdpi.com/2223-7747/12/13/2422
69Genome-wide identification of the mango pathogenesis-related 1 (PR1) gene family and functional analysis of MiPR1A genes in transgenic ArabidopsisSCIENTIA HORTICULTURAE4.3https://www.sciencedirect.com/science/article/pii/S0304423823004259
70Melatonin enhances KCl salinity tolerance by maintaining K+ homeostasis in Malus hupehensisPLANT BIOTECHNOLOGY JOURNAL13.8https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.14129
71Enhancing the Adaptability of Tea Plants (Camellia sinensis L.) to High-Temperature Stress with Small Peptides and BiosurfactantsPlants-Basel4.5https://www.mdpi.com/2223-7747/12/15/2817
72Synthesis of chitin nanocrystals supported Zn2+ with high activity against tobacco mosaic virusINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES8.2https://www.sciencedirect.com/science/article/pii/S0141813023030647
73Transcriptome and WGCNA reveal hub genes in sugarcane tiller seedlings in response to drought stressScientific Reports4.6https://www.nature.com/articles/s41598-023-40006-x
74Transcription Factor GmERF105 Negatively Regulates Salt Stress Tolerance in Arabidopsis thalianaPlants-Basel4.5https://www.mdpi.com/2223-7747/12/16/3007
75Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao ChicksAntibiotics-Basel4.8https://www.mdpi.com/2079-6382/12/9/1356
76Genome-Wide and 16S rRNA Sequencing-Based Analysis on the Health Effects of Lacticaseibacillus paracasei XLK401 on ChicksMicroorganisms4.5https://www.mdpi.com/2076-2607/11/9/2140
77Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporumFrontiers in Microbiology5.2https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623355/
78Genome-wide identification of Glutathione peroxidase (GPX) family genes and silencing TaGPX3.2A reduced disease resistance in wheatPLANT PHYSIOLOGY AND BIOCHEMISTRY6.5https://www.sciencedirect.com/science/article/pii/S0981942823006502
79OsSTS, a Novel Allele of Mitogen-Activated Protein Kinase Kinase 4 (OsMKK4), Controls Grain Size and Salt Tolerance in RiceRice5.5https://thericejournal.springeropen.com/articles/10.1186/s12284-023-00663-y
80Melatonin Alleviates Antimony Toxicity by Regulating the Antioxidant Response and Reducing Antimony Accumulation in Oryza sativa L.Antioxidants7https://www.mdpi.com/2076-3921/12/11/1917
81Genomic features of a plant growth-promoting endophytic Enterobacter cancerogenus JY65 dominant in microbiota of halophyte Suaeda salsaPLANT AND SOIL4.9https://link.springer.com/article/10.1007/s11104-023-06360-5
82Promotional Properties of ACC Deaminase-Producing Bacterial Strain DY1-3 and Its Enhancement of Maize Resistance to Salt and Drought StressesMicroorganisms4.5https://www.mdpi.com/2076-2607/11/11/2654
83Integrating transcriptome and physiological analyses to elucidate the molecular responses of sorghum to fluxofenim and metolachlor herbicidePESTICIDE BIOCHEMISTRY AND PHYSIOLOGY4.7https://www.sciencedirect.com/science/article/pii/S0048357523003577


1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?

     答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。

          “24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。


2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?

     答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;

           微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。


3、问:分光法试剂盒与微板法试剂盒是否能通用?

     答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!