一、产品简介：
谷胺酰胺合成酶（GS，EC 6.3.1.2）主要存在于植物中，是生物体内氨同化的关键酶之一，植物吸收的无机氮经硝酸还原酶（NR）和亚硝酸还原酶（NIR）还原成NH4+后，通过谷胺酰胺合成酶（GS）参与的GS/GOGAT途径才能进行氮素的同化和利用。
谷胺酰胺合成酶（GS）在ATP和Mg2+存在下，催化铵离子和谷氨酸合成谷氨酰胺；谷氨酰胺进一步转化为γ─谷氨酰基异羟肟酸，在酸性条件下形成的络合物在540nm处有最大吸收峰，进而得到谷胺酰胺合成酶（GS）的酶活性大小。
二、所需的仪器和用品：
可见分光光度计、1 mL玻璃比色皿（光径1cm）、水浴锅、台式离心机、可调式移液器、研钵、冰和蒸馏水。
1. Guangming Lou. 2021. FLOURY ENDOSPERM19 encoding a class I glutamine amidotransferase afects grain quality in rice. Molecular breeding. IF=2.6
2.Zaid Khan.2022.How Biochar Affects Nitrogen Assimilation and Dynamics by Interacting Soil and Plant Enzymatic Activities: Quantitative Assessment of 2 Years Potted Study in a Rapeseed-Soil System..IF=.6.627
3.Kebin Yang.2022.Nitrogen fertilization in bamboo forest accelerates the shoot growth and alters the lignification process in shoots.IF=6.449
4.Dao-Jun Guo.2021.Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5.IF=5.754
5.Tingting Yuan.2022.An Integrated Regulatory Network of mRNAs, microRNAs, and lncRNAs Involved in Nitrogen Metabolism of Moso Bamboo.IF=4.772
6.Mingyan Tang.2020.Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass.IF=4.556
7.Lou, Guangming.2021.FLOURY ENDOSPERM19 encoding a class I glutamine amidotransferase affects grain quality in rice.IF=2.589
8.Yahui Ji.2022.Carbon dots promoted soybean photosynthesis and amino acid biosynthesis under drought stress: Reactive oxygen species scavenging and nitrogen metabolism..IF=10.753
9.Ningning Zhang.2022.Novel Antidepressant Mechanism of Ginsenoside Rg1 in Regulating the Dysfunction of the Glutamatergic System in Astrocytes.IF=6.208
10.Xuan Hou.2023.Warming and microplastic pollution shape the carbon and nitrogen cycles of algae.IF=14.224
11.Yuchen Li.2023.Glycine-Induced Phosphorylation Plays a Pivotal Role in Energy Metabolism in Roots and Amino Acid Metabolism in Leaves of Tea Plant.IF=5.561
12.Shuqing Guo.2023.Concurrence of microplastics and heat waves reduces rice yields and disturbs the agroecosystem nitrogen cycle.IF=14.224
13.Cun Yu.2023.Trichoderma longibrachiatum Inoculation Improves Drought Resistance and Growth of Pinus massoniana Seedlings through Regulating Physiological Responses and Soil Microbial Community.IF=4.7
14.Xiaojian Qin.2023.Transcriptomic and Physiological Analyses of Two Rice Restorer Lines under Different Nitrogen Supplies Provide Novel Insights into Hybrid Rice Breeding.IF=4.5
1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？
答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。
2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？
答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;
微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。
3、问：分光法试剂盒与微板法试剂盒是否能通用？
答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！
