一、产品简介：

硝酸还原酶（NR，EC 1.7.1.3）广泛存在于植物中，是一种诱导酶。是植物硝态氮转化为氨态氮的关键酶，与作物有效吸收和利用氮素肥料有关，是植物营养或农田施肥的指标之一，也可作为品种选育的指标之一。

本试剂盒采用离体法检测，通过硝酸还原酶催化硝酸盐还原为亚硝酸盐，亚硝酸盐进一步与磺胺及α-萘胺定量生成紫红色偶氮化合物；颜色深浅与硝酸还原酶活性呈正比，通过检测该物质于530nm的吸光值，即可得出硝酸还原酶的活性。

二、所需的仪器和用品：

     可见分光光度计、1mL玻璃比色皿（光径1cm）、可调式移液器、水浴锅/恒温培养箱、台式离心机、研钵、冰和蒸馏水。

1. Ying Liang. 2022. The Interaction Between Nitrogen Supply and Light Quality Modulates Plant Growth and Resource Allocation. Frontiers in Plant Science. IF=5.47

2.Ying Liang.2022.The Interaction Between Nitrogen Supply and Light Quality Modulates Plant Growth and Resource Allocation.IF=6.627

3.Zaid Khan.2022.How Biochar Affects Nitrogen Assimilation and Dynamics by Interacting Soil and Plant Enzymatic Activities: Quantitative Assessment of 2 Years Potted Study in a Rapeseed-Soil System..IF=.6.627

4.Kebin Yang.2022.Nitrogen fertilization in bamboo forest accelerates the shoot growth and alters the lignification process in shoots.IF=6.449

5.Meng Tan.2022.Polyamines Metabolism Interacts with γ-Aminobutyric Acid, Proline and Nitrogen Metabolisms to Affect Drought Tolerance of Creeping Bentgrass.IF=6.208

6.Dao-Jun Guo.2021.Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5.IF=5.754

7.Tingting Yuan.2022.An Integrated Regulatory Network of mRNAs, microRNAs, and lncRNAs Involved in Nitrogen Metabolism of Moso Bamboo.IF=4.772

8.Yahui Ji.2022.Carbon dots promoted soybean photosynthesis and amino acid biosynthesis under drought stress: Reactive oxygen species scavenging and nitrogen metabolism..IF=10.753

9.Yuzhao Tao.2023.Effect of Mn Deficiency on Carbon and Nitrogen Metabolism of Different Genotypes Seedlings in Maize (Zea mays L.).IF=4.658

10.Shuqing Guo.2023.Concurrence of microplastics and heat waves reduces rice yields and disturbs the agroecosystem nitrogen cycle.IF=14.224

1、问：官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢？

     答：“24样”、“48样”、“96样”是试剂盒规格，我们定义了试剂盒可以测多少样，对于试剂盒需要的试剂量都给足的。

          “24样”、“48样”、“96样”规格的试剂盒，可以检测24个样、48个样、96个样；即分别得到24个、48个、96个数据。

2、问：官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别？

     答：分光法：指使用紫外可见分光光度计检测，若无紫外可见光分光度计，订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是：光径：1cm,容积：1mL, 狭缝宽3mm;

           微板法：指使用全波段连续酶标仪检测；若无全波段酶标仪，订购指标时务必咨询公司技术， 本公司微板法试剂盒内送96孔普通酶标板，客户无需另外购买耗材。

3、问：分光法试剂盒与微板法试剂盒是否能通用？

     答：公司针对用户实验室具备的实验仪器条件，做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样，结果可以通用，但是不同体系的试剂盒不可以相互混匀！