一、产品简介:
单脱氢抗坏血酸还原酶(MDHAR,EC 1.6.5.4 )是使抗坏血酸(AsA)再生的关键酶之一,催化MDHA重新还原成AsA,对于维持抗坏血酸的抗氧化特性具有重要作用。
MDHAR 催化NADH 还原MDHA 生成 AsA和NAD+,通过测定NADH在340 nm处的光吸收下降速率,来计算出MDHAR活性。
二、所需的仪器和用品:
紫外分光光度计、1mL石英比色皿(光径1cm)、研钵、冰、台式离心机、可调式移液枪、水浴锅。
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