一、产品简介:
纤维素是植物细胞壁的主要成分之一。纤维素含量的多少,关系到植物细胞组织发达程度,因而影响作物的抗倒伏、抗病虫害能力的强弱。
纤维素是由葡萄糖基组成的多糖,在酸性条件下加热使其水解成葡萄糖。然后在浓硫酸作用下,使单糖脱水生成糠醛类化合物。利用蒽酮试剂与糠醛类化合物反应生成蓝绿色物质。经光谱扫描该蓝绿色物质在620nm处有最大吸收峰,进而得到纤维素含量。
二、所需的仪器和用品:
酶标仪、96孔板、水浴锅、可调式移液器、80%乙醇、丙酮、浓硫酸、研钵和蒸馏水。
1. Fuhua Fan. 2021. Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings. International Journal of Molecular Sciences.IF=5.9
2. Xinyue Bi. 2022. A novel strategy for improving watermelon resistance to cucumber green mottle mosaic virus by exogenous boron application. Molecular Plant Pathology IF=5.66
3.Jiemeng Tao.2022.Metagenomic insight into the microbial degradation of organic compounds in fermented plant leaves.IF=8.431
4.Hong Yang.2022.Wheat Escapes Low Light Stress by Altering Pollination Types.IF=6.627
5.Fuhua Fan.2021.Exogenous Brassinosteroid Facilitates Xylem Development in Pinus massoniana Seedlings.IF=5.924
6.Xinyue Bi.2022.A novel strategy for improving watermelon resistance to cucumber green mottle mosaic virus by exogenous boron application.IF=5.663
7.Xinyue Bi.2022.Suppression of Cucumber Green Mottle Mosaic Virus Infection by Boron Application: From the Perspective of Nutrient Elements and Carbohydrates.IF=5.895
8.Qixian Wu.2022.Multiple metabolomics comparatively investigated the pulp breakdown of four dragon fruit cultivars during postharvest storage.IF=7.425
9.Fei Ren.2023.Deciphering Microbial Diversity and Functional Codes of Traditional Fermented Whole Grain Tianpei from Typical Regions of China.IF=5.123
10.Qingqing Gu.2023.Physiological and Full-Length Transcriptome Analyses Reveal the Dwarfing Regulation in Trifoliate Orange (Poncirus trifoliata L.).IF=4.658
11.Jia Wang.2023.Cell wall remodeling in woody tung oil tree root xylem contributes to deterrence of the Fusarium wilt pathogen.IF=6.449
12.Pengfei Wan.2023.Enhanced degradation of extracellular polymeric substances by yeast in activated sludge to achieve sludge reduction.IF=11.889
13.Mengyao Li.2023.Combined evaluation of agronomic and quality traits to explore heat germplasm in celery (Apium graveolens L.).IF=4.342
14.Wenqi Li.2023.Identification, pathogenic mechanism and control of Rhizopus oryzae causing postharvest fruit rot in pumpkin.IF=7
1、问:官网上试剂盒规格标注的“24样”、“48样”、“96样”是什么意思呢?
答:“24样”、“48样”、“96样”是试剂盒规格,我们定义了试剂盒可以测多少样,对于试剂盒需要的试剂量都给足的。
“24样”、“48样”、“96样”规格的试剂盒,可以检测24个样、48个样、96个样;即分别得到24个、48个、96个数据。
2、问:官网上试剂盒检测方法中"可见分光法/紫外分光法"与“微板法”是什么区别?
答:分光法:指使用紫外可见分光光度计检测,若无紫外可见光分光度计,订购时务必咨询公司技术。公司分光法试剂盒采用的比色皿规格是:光径:1cm,容积:1mL, 狭缝宽3mm;
微板法:指使用全波段连续酶标仪检测;若无全波段酶标仪,订购指标时务必咨询公司技术, 本公司微板法试剂盒内送96孔普通酶标板,客户无需另外购买耗材。
3、问:分光法试剂盒与微板法试剂盒是否能通用?
答:公司针对用户实验室具备的实验仪器条件,做了两个体系的试剂盒。两种体系试剂盒检测指标的原理一样,结果可以通用,但是不同体系的试剂盒不可以相互混匀!