一、产品简介：
磷酸酶是普遍存在的一种重要的水解酶，碱性磷酸酶（AKP，EC 3.1.3.1）是一种含锌的糖蛋白酶，在碱性环境中可水解各种天然及人工合成的磷脂单酯化合物。本试剂盒提供一种高灵敏度，简单，直接的检测方法，使用磷酸对硝基苯酯（pNPP）作为底物，生成黄色的产物PNP，该产物在405nm处有最大吸收峰。通过检测PNP在405nm下的增加速率，即可得到碱性磷酸酶（AKP）活性的大小。
二、所需的仪器和用品：
酶标仪、96孔板、低温离心机、水浴锅、可调式移液器和蒸馏水。
Chengze Yu.2021.Hydrothermal pretreatment contributes to accelerate maturity during the composting of lignocellulosic solid wastes.IF=9.624
Qin, Xiaomin.2022.Increased nodular P level induced by intercropping stimulated nodulation in soybean under phosphorus deficiency.IF=4.996
Yun-Ze Chen.2021.Transcriptomic analysis of interactions between Lymantria dispar larvae and carvacrol.IF=3.963
Nan Xu.2023.Lonicera japonica Thunb. as a promising antibacterial agent for Bacillus cereus ATCC14579 based on network pharmacology, metabolomics, and in vitro experiments.IF=4.036